Human CLEC4E Alexa Fluor® 405-conjugated Antibody

Recombinant Monoclonal Antibody
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Human CLEC4E Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Detects human CLEC4E in direct ELISAs.
Recombinant Monoclonal Rabbit IgG Clone # 2455C
Protein A or G purified from cell culture supernatant
Chinese Hamster Ovary cell line, CHO-derived recombinant human CLEC4E
Accession # Q9ULY5
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)


Recommended Concentration
Flow Cytometry
0.25-1 µg/106 cells
HEK293 Human Cell Line Transfected with Human CLEC4e and eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CLEC4E

CLEC4E, also known as Mincle, is an approximately 30 kDa type 2 transmembrane C-type lectin that functions as an activating innate immune receptor (1). Human CLEC4E consists of a 19 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD) that contains the C-type lectin domain (2). Within the ECD, human CLEC4E shares 65% and 68% aa sequence identity with mouse and rat CLEC4E, respectively. CLEC4E is expressed on monocytes, macrophages, and immature dendritic cells (2-5). It associates with CLEC4D/MCL and the gamma chain signaling subunits of Fc receptors (mediated by an Arg residue in the CLEC4E transmembrane segment) (3, 5, 6). Human CLEC4E binds to mycobacterial glycolipids including the immune adjuvant TDM (cord factor), its synthetic analog TDB, and GroMM (3, 4, 7-10). It also binds the nuclear protein SAP130 which can be released from necrotic cells (5) and cholesterol crystals deposited in atherosclerotic plaques (11). Mouse CLEC4E, in contrast, does not appear to interact with TDB, GroMM, or cholesterol crystals (7, 8, 11). CLEC4E ligation triggers phagocytosis and the production of inflammatory chemokines and cytokines (3-6, 8, 10). The fungus Fonsecaea monophora may evade immune clearance through binding to CLEC4E and suppressing IL-12 production and Th1 cell differentiation instead of promoting inflammation (9).

  1. Richardson, M.B. and S.J. Williams (2014) Front. Immunol. 5:288.
  2. Matsumoto, M. et al. (1999) J. Immunol. 163:5039.
  3. Ishikawa, E. et al. (2009) J. Exp. Med. 206:2879.
  4. Wells, C.A. et al. (2008) J. Immunol. 180:7404.
  5. Yamasaki, S. et al. (2008) Nat. Immunol. 9:1179.
  6. Lobato-Pascual, A. et al. (2013) Eur. J. Immunol. 43:3167.
  7. Hattori, Y. et al. (2014) J. Biol. Chem. 289:15405.
  8. Ostrop, J. et al. (2015) J. Immunol. 195:2417.
  9. Wevers, B.A. et al. (2014) Cell Host Microbe 15:494.
  10. Ishikawa, T. et al. (2013) Cell Host Microbe 13:477.
  11. Kiyotake, R. et al. (2015) J. Biol. Chem. 290:25322.
Long Name
C-type Lectin Domain Family 4, Member E
Entrez Gene IDs
26253 (Human); 56619 (Mouse); 450223 (Rat)
Alternate Names
CLEC4E; CLECSF9; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 9; C-type lectin domain family 4 member E; C-type lectin domain family 4, member E; Macrophage-inducible C-type lectin; MINCLE; MINCLEC-type lectin superfamily member 9

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Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.


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