Human CX3CL1/Fractalkine XL Magnetic Luminex® Performance Assay

Name: Human CX3CL1/Fractalkine XL Magnetic Luminex® Performance Assay
Brand: R&D Systems
SKU: LUXLM362
Price: 165 USD

Catalog #: LUXLM362 Datasheet / COA / SDS

Discontinued Product

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Human CX3CL1/Fractalkine XL Magnetic Luminex® Performance Assay Summary

Assay Type	bead-based multiplex assay for the Luminex^® platform
Format	1 x 96-well microplate and antibody-coated beads
Analytes Detected	Please see analyte list in assay customization tool below.
Performance Validation	Luminex Assays are validated for use with cell culture supernatants, plasma and serum. All Luminex assays are tested for sensitivity, intra-assay precision, inter-assay precision and to ensure assay linearity for validated sample types. Antibody pairs are selected and tested to confirm the parallel detection of natural and recombinant standard protein and to ensure the accurate determination of target analytes within biological samples. Assays for each target analyte are screened against all target analytes to confirm low antibody cross-reactivity. View Luminex High Performance Human XL validation data on rndsystems.com.

Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services.

Precision

	Intra-Assay Precision		Inter-Assay Precision
Intra-assay Precision (precision within an assay) - Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (precision between assays) - Three samples of known concentration were tested in twenty-five separate assays to assess precision between assays.
Sample	1	2	1	2
n	20	20	25	25
Mean (pg/mL)	851	29,972	806	30,803
Standard deviation	31.2	537	126	4060
CV (%)	3.7	1.8	15.6	13.2

Recovery

Sample Type	Average % Recovery	Range (%)
Samples containing and/or spiked with human Fractalkine were evaluated for recovery.
Cell culture supernate	105	96-127
Serum	88	69-120
EDTA plasma	94	71-132
Heparin plasma	99	74-142

Linearity

		Cell culture supernate	Serum	EDTA plasma	Heparin plasma
Samples containing and/or spiked with human Fractalkine were serially diluted to evaluate assay linearity.
1:2	Average % of Expected	98	123	106	100
1:2	Range (%)	97-99	115-130	103-109	98-102
1:4	Average % of Expected	101	120	118	124
1:4	Range (%)	97-103	112-135	112-124	114-137
1:8	Average % of Expected	103	125	122	129
1:8	Range (%)	96-107	111-142	115-129	116-146

Which Luminex Assay is right for me?

Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.

Background: CX3CL1/Fractalkine

CX3CL1/Fractalkine is a transmembrane adhesion protein with a chemokine domain separated from the membrane by a mucin stalk. It is upregulated on many cell types during inflammation. The chemokine and mucin regions can be shed as a soluble chemokine that signals through the receptor CX3CR1. During extravasation, membrane-bound CX3CL1 traps leukocytes, then is cleaved to allow diapedesis. Soluble CX3CL1 protects neurons from microglial neurotoxicity, recruits macrophages for wound healing, recruits osteoclast precursors for bone resorption, and contributes to pathogenesis in coronary artery disease.

Entrez Gene IDs

6376 (Human); 20312 (Mouse); 89808 (Rat); 102117496 (Cynomolgus Monkey)

Alternate Names

ABCD-3; C3Xkine; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CX3CL1; CXC3; CXC3C; FKN; Fractalkine; Neurotactin; neurotactin); NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine; NTTSmall-inducible cytokine D1; SCYD1C-X3-C motif chemokine 1; small inducible cytokine subfamily D (Cys-X3-Cys), member-1