Human CXCL1/GRO alpha Quantikine ELISA Kit

Newer Version Available: DGR00B
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DGR00 has been discontinued and is replaced by DGR00B.

Human GRO alpha  ELISA Cell Culture Supernate Standard Curve
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Citations (43)
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Human CXCL1/GRO alpha Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
2.75-4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 uL), Serum (200 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL), Citrate Plasma (200 uL)
Sensitivity
10 pg/mL
Assay Range
31.3 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Specificity
Natural and recombinant human GRO alpha
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine® Human CXCL1/GRO alpha Immunoassay is a 2.75-4.5 hour solid phase ELISA designed to measure human GRO alpha in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human GRO alpha and antibodies raised against the recombinant protein. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural human GRO alpha showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human GRO alpha.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 81.4 318 734 87.4 320 758
Standard Deviation 3.7 7.4 55 11.9 27.6 62.7
CV% 4.5 2.3 7.5 13.6 8.6 8.3

Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 61.4 251 736 62.8 252 734
Standard Deviation 2.2 6 20 3.6 11.9 38.9
CV% 3.6 2.4 2.7 5.7 4.7 5.3

Recovery

The recovery of human GRO alpha spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 97 81-108
Citrate Plasma (n=10) 90 80-98
EDTA Plasma (n=10) 93 86-102
Heparin Plasma (n=10) 95 83-109
Serum (n=10) 98 87-108

Linearity

To assess linearity of the assay, samples containing and/or spiked with high concentrations of human GRO alpha were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human GRO alpha  ELISA Linearity
Human CXCL1/GRO alpha/KC/CINC-1 ELISA Linearity

Scientific Data

Human GRO alpha ELISA Cell Culture Supernate Standard Curve

Human CXCL1/GRO alpha/KC/CINC-1 ELISA Cell Culture Supernate Standard Curve

Human CXCL1/GRO alpha/KC/CINC-1 ELISA Serum/Plasma Standard Curve

Human GRO alpha ELISA Serum/Plasma Standard Curve

Product Datasheets

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Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL1/GRO alpha/KC/CINC-1

CXCL1/GRO alpha, also known as KC in mouse and CINC-1 in rat, is a member of the CXC family of chemokines. Human CXCL1/GRO alpha is 107 amino acids (aa) in length with a predicted molecular weight of 11 kDa. The mouse and rat orthologs share 57% and 59% amino acid sequence identity with the human protein, respectively. Genome-wide analysis suggests that the protein encoded by the mouse Cxcl3 gene is homologous to human CXCL1/GRO alpha protein. Human CXCL1/GRO alpha can be cleaved into three isoforms CXCL1/GRO alpha (aa 4-73), CXCL1/GRO alpha (aa 5-73), and CXCL1/GRO alpha (aa 6-73) which show higher activity than the full length protein. CXCL1/GRO alpha is secreted by mast cells and macrophages whereupon it acts as a chemoattractant for neutrophils.

Entrez Gene IDs:
2919 (Human); 14825 (Mouse); 81503 (Rat)
Alternate Names:
CINC1; CINC-1; CXCL1; FSP; GRO alpha; GRO1; GROa; KC; MGSA; MGSA-a; MGSA-alpha; NAP-3; SCYB1

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent for Serum & Plasma Samples Only
  4.   For Serum & Plasma Samples Only: Add 50 µL of Assay Diluent to each well.

  5. 200 µL Standard, Control, or Sample
  6.   Add 200 µL of Standard, control, or sample to each well.
  7.   For Serum & Plasma Samples: Cover with a plate sealer, and incubate at room temperature for 2 hours.
    For Cell Culture Supernate Samples: Cover with a plate sealer, and incubate at room temperature for 1.5 hours.
  8.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.

  9. 200 µL Conjugate
  10.   Add 200 µL of Conjugate to each well.
  11.   For Serum & Plasma Samples: Cover with a new plate sealer, and incubate at 2-8 °C for 2 hours.
    For Cell Culture Supernate Samples: Cover with a new plate sealer, and incubate at 2-8 °C for 1 hour.
  12.   Aspirate and wash 3 times.

  13. 200 µL Substrate Solution
  14. Add 200 µL Substrate Solution to each well.
  15. For Serum & Plasma Samples: Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    For Cell Culture Supernate Samples: Incubate at room temperature for 15 minutes. PROTECT FROM LIGHT.

  16. 50 µL Stop Solution
  17. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human CXCL1/GRO alpha Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

43 Citations: Showing 1 - 10
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  1. Proof-of-Principle Study Suggesting Potential Anti-Inflammatory Activity of Butyrate and Propionate in Periodontal Cells
    Authors: AFP Santos, LCC Cervantes, L Panahipour, FÁ Souza, R Gruber
    International Journal of Molecular Sciences, 2022;23(19):.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. CXCL1-CXCR2 signalling mediates hypertensive retinopathy by inducing macrophage infiltration
    Authors: S Wang, J Bai, YL Zhang, QY Lin, X Han, WK Qu, PF Zhang, YS Ge, Q Zhao, HH Li
    Redox Biology, 2022;56(0):102438.
    Species: Human
    Sample Types: Serum
  3. Senescent Human Pancreatic Stellate Cells Secrete CXCR2 Agonist CXCLs to Promote Proliferation and Migration of Human Pancreatic Cancer AsPC-1 and MIAPaCa-2 Cell Lines
    Authors: T Takikawa, S Hamada, R Matsumoto, Y Tanaka, F Kataoka, A Sasaki, A Masamune
    International Journal of Molecular Sciences, 2022;23(16):.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. KIF4A promotes tumor progression of bladder cancer via CXCL5 dependent myeloid-derived suppressor cells recruitment
    Authors: N Lin, L Chen, Y Zhang, Y Yang, L Zhang, L Chen, P Zhang, H Su, M Yin
    Scientific Reports, 2022;12(1):6015.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Efficacy of chitinase-3-like protein 1 as an in�vivo bone formation predictable marker of maxillary/mandibular bone marrow stromal cells
    Authors: N Komabashir, F Suehiro, M Ishii, M Nishimura
    Regenerative Therapy, 2021;18(0):38-50.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Visfatin Enhances Breast Cancer Progression through CXCL1 Induction in Tumor-Associated Macrophages
    Authors: YY Wang, HD Chen, S Lo, YK Chen, YC Huang, SC Hu, YC Hsieh, AC Hung, MF Hou, SF Yuan
    Cancers, 2020;12(12):.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Monoclonal Antibody against CXCL1 (HL2401) as a Novel Agent in Suppressing IL6 Expression and Tumoral Growth
    Authors: M Miyake, H Furuya, S Onishi, K Hokutan, S Anai, O Chan, S Shi, K Fujimoto, S Goodison, W Cai, CJ Rosser
    Theranostics, 2019;9(3):853-867.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. The intrathecal expression and pathogenetic role of Th17 cytokines and CXCR2-binding chemokines in tick-borne encephalitis
    Authors: S Grygorczuk, R ?wierzbi?s, M Kondrusik, J Dunaj, P Czupryna, A Moniuszko, A Siemieniak, S Pancewicz
    J Neuroinflammation, 2018;15(1):115.
    Species: Human
    Sample Types: Serum
  9. Inflammatory cytokine production in tumor cells upon chemotherapy drug exposure or upon selection for drug resistance
    Authors: DW Edwardson, J Boudreau, J Mapletoft, C Lanner, AT Kovala, AM Parissenti
    PLoS ONE, 2017;12(9):e0183662.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. CAF-secreted CXCL1 conferred radioresistance by regulating DNA damage response in a ROS-dependent manner in esophageal squamous cell carcinoma
    Authors: H Zhang, J Yue, Z Jiang, R Zhou, R Xie, Y Xu, S Wu
    Cell Death Dis, 2017;8(5):e2790.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. CXCL1 is critical for pre-metastatic niche formation and metastasis in colorectal cancer
    Authors: D Wang, H Sun, J Wei, B Cen, RN DuBois
    Cancer Res., 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  12. MicroRNAs regulate cytokine responses in gingival epithelial cells
    Infect Immun, 2016;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. p53 and NF-kappaB coregulate proinflammatory gene responses in human macrophages.
    Authors: Lowe J, Menendez D, Bushel P, Shatz M, Kirk E, Troester M, Garantziotis S, Fessler M, Resnick M
    Cancer Res, 2016;74(8):2182-92.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. High salt primes a specific activation state of macrophages, M(Na).
    Authors: Zhang W, Zheng X, DU L, Sun J, Shen Z, Shi C, Sun S, Zhang Z, Chen X, Qin M, Liu X, Tao J, Jia L, Fan H, Zhou B, Yu Y, Ying H, Hui L, Liu X, Yi X, Liu X, Zhang L, Duan S
    Cell Res, 2015;25(8):893-910.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Stromal cells positively and negatively modulate the growth of cancer cells: stimulation via the PGE2-TNFalpha-IL-6 pathway and inhibition via secreted GAPDH-E-cadherin interaction.
    Authors: Kawada M, Inoue H, Ohba S, Yoshida J, Masuda T, Yamasaki M, Usami I, Sakamoto S, Abe H, Watanabe T, Yamori T, Shibasaki M, Nomoto A
    PLoS ONE, 2015;10(3):e0119415.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. Phosphoinositide 3-kinase inhibition restores neutrophil accuracy in the elderly: toward targeted treatments for immunosenescence.
    Authors: Sapey E, Greenwood H, Walton G, Mann E, Love A, Aaronson N, Insall R, Stockley R, Lord J
    Blood, 2014;123(2):239-48.
    Species: Human
    Sample Types: Sputum
  17. Targeted gene silencing using a follicle-stimulating hormone peptide-conjugated nanoparticle system improves its specificity and efficacy in ovarian clear cell carcinoma in vitro.
    Authors: Hong S, Zhang X, Chen J, Zhou J, Zheng Y, Xu C
    J Ovarian Res, 2013;6(1):80.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Influence of the CXCL1 rs4074 A allele on alcohol induced cirrhosis and HCC in patients of European descent.
    Authors: Nischalke H, Berger C, Lutz P, Langhans B, Wolter F, Eisenhardt M, Kramer B, Kokordelis P, Glassner A, Muller T, Rosendahl J, Fischer J, Berg T, Grunhage F, Leifeld L, Soyka M, Nattermann J, Sauerbruch T, Stickel F, Spengler U
    PLoS ONE, 2013;8(11):e80848.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Circulating levels of transforming growth factor-betaeta (TGF-beta) and chemokine (C-X-C motif) ligand-1 (CXCL1) as predictors of distant seeding of circulating tumor cells in patients with metastatic breast cancer.
    Authors: Divella R, Daniele A, Savino E, Palma F, Bellizzi A, Giotta F, Simone G, Lioce M, Quaranta M, Paradiso A, Mazzocca A
    Anticancer Res, 2013;33(4):1491-7.
    Species: Human
    Sample Types: Plasma
  20. Role of interleukin-32 in Helicobacter pylori-induced gastric inflammation.
    Authors: Sakitani K, Hirata Y, Hayakawa Y, Serizawa T, Nakata W, Takahashi R, Kinoshita H, Sakamoto K, Nakagawa H, Akanuma M, Yoshida H, Maeda S, Koike K
    Infect Immun, 2012;80(11):3795-803.
    Species: Human
    Sample Types: Cell Lysates
  21. A phase I trial of bortezomib with temozolomide in patients with advanced melanoma: toxicities, antitumor effects, and modulation of therapeutic targets.
    Authors: Su Y, Amiri KI, Horton LW
    Clin. Cancer Res., 2010;16(1):348-57.
    Species: Human
    Sample Types: Serum
  22. Thymic stromal lymphopoietin induces chemotactic and prosurvival effects in eosinophils: implications in allergic inflammation.
    Authors: Wong CK, Hu S, Cheung PF
    Am. J. Respir. Cell Mol. Biol., 2010;43(3):305-15.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. CXCR2 promotes ovarian cancer growth through dysregulated cell cycle, diminished apoptosis, and enhanced angiogenesis.
    Authors: Yang G, Rosen DG, Liu G
    Clin. Cancer Res., 2010;16(15):3875-86.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. Immunomodulation of nasal epithelial cells by Staphylococcus aureus-derived serine proteases.
    Authors: Rudack C, Sachse F, Albert N, Becker K, von Eiff C
    J. Immunol., 2009;183(11):7592-601.
    Species: Human
    Sample Types: Cell Culture Supernates
  25. Dysregulation of stromal derived factor 1/CXCR4 axis in the megakaryocytic lineage in essential thrombocythemia.
    Authors: Salim JP, Goette NP, Lev PR, Chazarreta CD, Heller PG, Alvarez C, Molinas FC, Marta RF
    Br. J. Haematol., 2009;144(1):69-77.
    Species: Human
    Sample Types: Plasma
  26. LPS induces GROalpha chemokine production via NF-kappaB in oral fibroblasts.
    Authors: Jonsson D, Amisten S, Bratthall G, Holm A, Nilsson BO
    Inflamm. Res., 2009;58(11):791-6.
    Species: Human
    Sample Types: Cell Culture Supernates
  27. IL-8 as antibody therapeutic target in inflammatory diseases: reduction of clinical activity in palmoplantar pustulosis.
    Authors: Skov L, Beurskens FJ, Zachariae CO, Reitamo S, Teeling J, Satijn D, Knudsen KM, Boot EP, Hudson D, Baadsgaard O, Parren PW, van de Winkel JG
    J. Immunol., 2008;181(1):669-79.
    Species: Human
    Sample Types: Complex Sample Type
  28. Duffy antigen modifies the chemokine response in human endotoxemia.
    Authors: Mayr FB, Spiel AO, Leitner JM, Firbas C, Kliegel T, Jilma-Stohlawetz P, Derendorf H, Jilma B
    Crit. Care Med., 2008;36(1):159-65.
    Species: Human
    Sample Types: Plasma
  29. The human IL-17F/IL-17A heterodimeric cytokine signals through the IL-17RA/IL-17RC receptor complex.
    Authors: Wright JF, Bennett F, Li B, Brooks J, Luxenberg DP, Whitters MJ, Tomkinson KN, Fitz LJ, Wolfman NM, Collins M, Dunussi-Joannopoulos K, Chatterjee-Kishore M, Carreno BM
    J. Immunol., 2008;181(4):2799-805.
    Species: Human
    Sample Types: Cell Culture Supernates
  30. A potential role of the CXC chemokine GROalpha in atherosclerosis and plaque destabilization: downregulatory effects of statins.
    Authors: Breland UM, Halvorsen B, Hol J, ØIe E, Paulsson-Berne G, Yndestad A, Smith C, Otterdal K, Hedin U, Waehre T, Sandberg WJ, Froland SS, Haraldsen G, Gullestad L, Damas JK, Hansson GK, Aukrust P
    Arterioscler. Thromb. Vasc. Biol., 2008;28(5):1005-11.
    Species: Human
    Sample Types: Plasma
  31. MMP-12 induces IL-8/CXCL8 secretion through EGFR and ERK1/2 activation in epithelial cells.
    Authors: Le Quement C, Guenon I, Gillon JY, Lagente V, Boichot E
    Am. J. Physiol. Lung Cell Mol. Physiol., 2008;294(6):L1076-84.
    Species: Human
    Sample Types: Cell Culture Supernates
  32. CT20126, a novel immunosuppressant, prevents collagen-induced arthritis through the down-regulation of inflammatory gene expression by inhibiting NF-kappaB activation.
    Authors: Lee SJ, Nam WD, Na HJ, Cho YL, Ha KS, Hwang JY, Lee H, Kim SO, Kwon YG, Kim YM
    Biochem. Pharmacol., 2008;76(1):79-90.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. Proliferation, differentiation, and cytokine secretion of human umbilical cord blood-derived mononuclear cells in vitro.
    Authors: Neuhoff S, Moers J, Rieks M, Grunwald T, Jensen A, Dermietzel R, Meier C
    Exp. Hematol., 2007;35(7):1119-31.
    Species: Human
    Sample Types: Cell Culture Supernates
  34. L5, the most electronegative subfraction of plasma LDL, induces endothelial vascular cell adhesion molecule 1 and CXC chemokines, which mediate mononuclear leukocyte adhesion.
    Authors: Abe Y, Fornage M, Yang CY, Bui-Thanh NA, Wise V, Chen HH, Rangaraj G, Ballantyne CM
    Atherosclerosis, 2007;192(1):56-66.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. Role of interleukin-8 and growth-regulated oncogene-alpha in the chemotactic migration of all-trans retinoic acid-treated promyelocytic leukemic cells toward alveolar epithelial cells.
    Authors: Tsai WH, Hsu HC, Lin CC, Ho CK, Kou YR
    Crit. Care Med., 2007;35(3):879-85.
    Species: Human
    Sample Types: Cell Culture Supernates
  36. Th1/Th2 cytokines reciprocally regulate in vitro pulmonary angiogenesis via CXC chemokine synthesis.
    Authors: Matsuda A, Fukuda S, Matsumoto K, Saito H
    Am. J. Respir. Cell Mol. Biol., 2007;38(2):168-75.
    Species: Human
    Sample Types: Cell Culture Supernates
  37. Different angiogenic activity in pulmonary sarcoidosis and idiopathic pulmonary fibrosis.
    Authors: Antoniou KM, Tzouvelekis A, Alexandrakis MG, Sfiridaki K, Tsiligianni I, Rachiotis G, Tzanakis N, Bouros D, Milic-Emili J, Siafakas NM
    Chest, 2006;130(4):982-8.
    Species: Human
    Sample Types: BALF
  38. Exhaled breath condensate levels of 8-isoprostane, growth related oncogene alpha and monocyte chemoattractant protein-1 in patients with chronic obstructive pulmonary disease.
    Authors: Ko FW, Lau CY, Leung TF, Wong GW, Lam CW, Hui DS
    Respir Med, 2006;100(4):630-8.
    Species: Human
    Sample Types: Exhaled Breath Condensate (EBC)
  39. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
    Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
    J. Immunol., 2005;175(9):6177-89.
    Species: Human
    Sample Types: Cell Culture Supernates
  40. ELR+ CXC chemokines in human milk.
    Authors: Maheshwari A, Christensen RD, Calhoun DA
    Cytokine, 2003;24(3):91-102.
    Species: Human
    Sample Types: Serum
  41. TNFalpha-induced macrophage chemokine secretion is more dependent on NF-kappaB expression than lipopolysaccharides-induced macrophage chemokine secretion.
    Authors: Ciesielski CJ, Andreakos E, Foxwell BM, Feldmann M
    Eur. J. Immunol., 2002;32(7):2037-45.
    Species: Human
    Sample Types: Cell Culture Supernates
  42. Il-6 and its soluble receptor orchestrate a temporal switch in the pattern of leukocyte recruitment seen during acute inflammation.
    Authors: Hurst SM, Wilkinson TS, McLoughlin RM, Jones S, Horiuchi S, Yamamoto N, Rose-John S, Fuller GM, Topley N, Jones SA
    Immunity, 2001;14(6):705-14.
    Species: Human
    Sample Types: Cell Culture Supernates
  43. High EMT Signature Score of Invasive Non-Small Cell Lung Cancer (NSCLC) Cells Correlates with NFkappaB Driven Colony-Stimulating Factor 2 (CSF2/GM-CSF) Secretion by Neighboring Stromal Fibroblasts.
    Authors: Rudisch A, Dewhurst M, Horga L, Kramer N, Harrer N, Dong M, van der Kuip H, Wernitznig A, Bernthaler A, Dolznig H, Sommergruber W
    PLoS ONE, 0;10(4):e0124283.
    Species: Human
    Sample Types: Cell Culture Supernates

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Human CXCL1/GRO alpha Quantikine ELISA Kit
By Anonymous on 09/14/2019
Sample Tested: Recombinant protein