CXCL1/GRO alpha/KC/CINC-1 is an ELR+ angiogenic chemokine that interacts with CXCR2/IL-8 RB to attract and activate neutrophils. It is produced at sites of microbial infection by macrophages, monocytes, Kupffer cells, hepatocytes, endothelial and epithelial cells, cardiac and vascular smooth muscle cells, fibroblasts, melanocytes, mast cells, and neurons. CXCL1 function is associated with the development and extent of atherosclerosis, Alzheimer’s disease, and tumor angiogenesis.
Human CXCL1/GRO alpha Quantikine ELISA Kit
R&D Systems | Catalog # DGR00
Discontinued Product
DGR00 has been discontinued.
An alternative/replacement product is available:
DGR00B. View all CXCL1/GRO alpha/KC/CINC-1 products.
Key Product Details
Assay Length
2.75-4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 µL), Serum (200 µL), EDTA Plasma (200 µL), Heparin Plasma (200 µL), Citrate Plasma (200 µL)
Sensitivity
10 pg/mL
Assay Range
31.3-1000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
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Product Summary for Human CXCL1/GRO alpha Quantikine ELISA Kit
The Quantikine® Human CXCL1/GRO alpha Immunoassay is a 2.75-4.5 hour solid phase ELISA designed to measure human GRO alpha in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human GRO alpha and antibodies raised against the recombinant protein. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural human GRO alpha showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human GRO alpha.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Human
Specificity
Natural and recombinant human GRO alpha
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 81.4 | 318 | 734 | 87.4 | 320 | 758 |
| Standard Deviation | 3.7 | 7.4 | 55.0 | 11.9 | 27.6 | 62.7 |
| CV% | 4.5 | 2.3 | 7.5 | 13.6 | 8.6 | 8.3 |
Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 61.4 | 251 | 736 | 62.8 | 252 | 734 |
| Standard Deviation | 2.2 | 6.0 | 20.0 | 3.6 | 11.9 | 38.9 |
| CV% | 3.6 | 2.4 | 2.7 | 5.7 | 4.7 | 5.3 |
Recovery for Human CXCL1/GRO alpha Quantikine ELISA Kit
The recovery of human GRO alpha spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 97 | 81-108 |
| Citrate Plasma (n=10) | 90 | 80-98 |
| EDTA Plasma (n=10) | 93 | 86-102 |
| Heparin Plasma (n=10) | 95 | 83-109 |
| Serum (n=10) | 98 | 87-108 |
Linearity
To assess linearity of the assay, samples containing and/or spiked with high concentrations of human GRO alpha were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human CXCL1/GRO alpha Quantikine ELISA Kit
Human GRO alpha ELISA Cell Culture Supernate Standard Curve
Human CXCL1/GRO alpha/KC/CINC-1 ELISA Cell Culture Supernate Standard Curve
Human CXCL1/GRO alpha/KC/CINC-1 ELISA Serum/Plasma Standard Curve
Human GRO alpha ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CXCL1/GRO alpha/KC/CINC-1
Alternate Names
CINC-1, CINC1, GRO alpha, KC, MGSA-alpha
Gene Symbol
CXCL1
Additional CXCL1/GRO alpha/KC/CINC-1 Products
Product Documents for Human CXCL1/GRO alpha Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human CXCL1/GRO alpha Quantikine ELISA Kit
For research use only
Related Research Areas
Citations for Human CXCL1/GRO alpha Quantikine ELISA Kit
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Protocols
View specific protocols for Human CXCL1/GRO alpha Quantikine ELISA Kit (DGR00):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- For Serum & Plasma Samples Only: Add 50 µL of Assay Diluent to each well.
- Add 200 µL of Standard, control, or sample to each well.
- For Serum & Plasma Samples: Cover with a plate sealer, and incubate at room temperature for 2 hours.
For Cell Culture Supernate Samples: Cover with a plate sealer, and incubate at room temperature for 1.5 hours. - Aspirate each well and wash, repeating the process twice for a total of 3 washes.
- Add 200 µL of Conjugate to each well.
- For Serum & Plasma Samples: Cover with a new plate sealer, and incubate at 2-8 °C for 2 hours.
For Cell Culture Supernate Samples: Cover with a new plate sealer, and incubate at 2-8 °C for 1 hour. - Aspirate and wash 3 times.
- Add 200 µL Substrate Solution to each well.
- For Serum & Plasma Samples: Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
For Cell Culture Supernate Samples: Incubate at room temperature for 15 minutes. PROTECT FROM LIGHT. - Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
50 µL Assay Diluent for Serum & Plasma Samples Only
200 µL Standard, Control, or Sample
200 µL Conjugate
200 µL Substrate Solution
50 µL Stop Solution
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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