Human CXCL16 APC-conjugated Antibody

Catalog #: FAB976A
1 Citation Datasheet / COA / SDS

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FAB976A

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Detection of CXCL16 in Human Denditic Cells by Flow Cytometry.

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Human CXCL16 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CXCL16 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human CXCL1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12/SDF-1 alpha,12/SDF-1 beta, 13, recombinant mouse CXCL1, 2, 6, 9, 10, 12/SDF-1 alpha, 13, 16, recombinant rat CXCL1, 2, 3/CINC-2 alpha, 3/CINC-2 beta, recombinant canine CXCL8, or recombinant porcine CXCL8 is observed.

Source

Monoclonal Rat IgG_2A Clone # 256213

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human CXCL16
Asn49-Pro137
Accession # NP_071342

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CXCL16 in Human Denditic Cells by Flow Cytometry. Human monocyte-derived dendritic cells were stained with Rat Anti-Human CXCL16 APC-conjugated Monoclonal Antibody (Catalog # FAB976A) and Mouse Anti-Human CD83 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1774F). Quadrant markers were set based on control antibody staining (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CXCL16

CXCL16 is a transmembrane protein with an extracellular domain consisting of a chemokine domain suspended on a mucin-like stalk. It functions as a ligand for the HIV co‑receptor, CXCR6 (Bonzo).

Entrez Gene IDs

58191 (Human); 66102 (Mouse)

Alternate Names

chemokine (C-X-C motif) ligand 16; CXC chemokine ligand 16; CXCL16; CXCLG16; Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; SCYB16; Small-inducible cytokine B16; SRPSOXC-X-C motif chemokine 16; SR-PSOXTransmembrane chemokine CXCL16

Product Datasheets

Product Datasheet

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Related Research Areas

Citation for Human CXCL16 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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