Human CXCL16 APC-conjugated Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CXCL16 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human CXCL1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12/SDF-1 alpha,12/SDF-1 beta, 13, recombinant mouse CXCL1, 2, 6, 9, 10, 12/SDF-1 alpha, 13, 16, recombinant rat CXCL1, 2, 3/CINC-2 alpha, 3/CINC-2 beta, recombinant canine CXCL8, or recombinant porcine CXCL8 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 256213
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human CXCL16
    Asn49-Pro137
    Accession # NP_071342
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CXCL16 in Human Denditic Cells by Flow Cytometry. Human monocyte-derived dendritic cells were stained with Rat Anti-Human CXCL16 APC‑conjugated Monoclonal Antibody (Catalog # FAB976A) and Mouse Anti-Human CD83 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB1774F). Quadrant markers were set based on control antibody staining (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CXCL16

CXCL16 is a transmembrane protein with an extracellular domain consisting of a chemokine domain suspended on a mucin-like stalk. It functions as a ligand for the HIV co‑receptor, CXCR6 (Bonzo).

  • Entrez Gene IDs:
    58191 (Human); 66102 (Mouse)
  • Alternate Names:
    chemokine (C-X-C motif) ligand 16; CXC chemokine ligand 16; CXCL16; CXCLG16; Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; SCYB16; Small-inducible cytokine B16; SRPSOXC-X-C motif chemokine 16; SR-PSOXTransmembrane chemokine CXCL16
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. ADAM10-Interacting Tetraspanins Tspan5 and Tspan17 Regulate VE-Cadherin Expression and Promote T Lymphocyte Transmigration
    Authors: JS Reyat, M Chimen, PJ Noy, J Szyroka, GE Rainger, MG Tomlinson
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Allophycocyanin Isotype Control

Ctrl IC006A 7  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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