Human/Cynomolgus Monkey PD-L1/B7-H1 QuicKit ELISA New

Catalog #: QK1146 Datasheet / COA / SDS

Human/Cynomolgus Monkey PD-L1/B7-H1 QuicKit ELISA

Catalog #	Availability	Size / Price	Qty
QK1146
SK1146
PK1146

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QC325: QuicKit Control for Human/Cynomolgus Monkey PD-L1/B7-H1

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Human/Cynomolgus Monkey PD-L1/B7-H1 QuicKit ELISA Standard Curve

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human/Cynomolgus Monkey PD-L1/B7-H1 QuicKit ELISA Summary

Assay Length

80 minutes

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 uL), Cell Lysates (100 uL), Cynomolgus Monkey EDTA Plasma (100 uL), Cynomolgus Monkey Serum (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Urine (100 uL)

Sensitivity

2.29 pg/mL

Assay Range

14.1 - 900 pg/mL (Cell Culture Supernates, Cell Lysates, Cynomolgus Monkey EDTA Plasma, Cynomolgus Monkey Serum, Serum, EDTA Plasma, Heparin Plasma, Urine)

Specificity

Natural and recombinant human PD-L1/B7-H1 and natural cynomolgus monkey PD-L1/B7-H1.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine QuicKit Human/Cynomolgus Monkey PD-L1/B7-H1 Immunoassay is a one-step, 80-minute solid phase ELISA designed to measure human/cynomolgus monkey PD-L1/B7-H1 levels in cell culture supernates, serum, plasma, cell lysates, and urine. It contains recombinant human PD-L1/B7-H1 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human/cynomolgus monkey PD-L1/B7-H1 showed linear curves that were parallel to the standard curves obtained using the recombinant QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural human/cynomolgus monkey PD-L1/B7-H1.

Precision

Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least five technicians

Cell Culture Supernates, Cell Lysates, Cynomolgus Monkey EDTA Plasma, Cynomolgus Monkey Serum, Serum, EDTA Plasma, Heparin Plasma, Urine

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	20	20	10	10
Mean (pg/mL)	86.8	516	89.1	557
Standard Deviation	2.39	9.1	11.6	73.9
CV%	2.8	1.8	13	13.3

Recovery

The recovery of PD-L1/B7-H1 is spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	108	105-111
Cynomolgus Monkey EDTA Plasma (n=2)	113	108-119
Cynomolgus Monkey Serum (n=2)	103	97-109
EDTA Plasma (n=2)	104	99-114
Heparin Plasma (n=2)	98	91-108
Lysis Buffer (n=1)	121	117-127
Serum (n=2)	103	96-110
Urine (n=2)	110	106-116

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of PD-L1/B7-H1 were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Cynomolgus Monkey PD-L1/B7-H1 QuicKit ELISA Linearity

Scientific Data

N/A

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Human/Cynomolgus Monkey PD-L1/B7-H1 QuicKit ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PD-L1/B7-H1

PD-L1, also known as B7-H1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells, keratinocytes, enothelial and intestinal epithelial cells, as well as a variety of carcinomas and melanoma. PD-L1 binds to T cell B7-1/CD80 and PD-1. It suppresses T cell activation and proliferation and induces the apoptosis of activated T cells. It plays a role in the development of immune tolerance by promoting T cell anergy and enhancing regulatory T cell development. PD-L1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells and inhibits the development of Th17 cells. In cancer, PD-L1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells.

Long Name:

Programmed Death Ligand 1

Entrez Gene IDs:

29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)

Alternate Names:

Avelumab; B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDCD1LG1MGC142296; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1