Human DC-SIGN+DC-SIGNR Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB16211R-100UG
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Human DC-SIGN+DC-SIGNR Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human DC‑SIGN in direct ELISAs and Western blots. Was reported to cross-react with human DC‑SIGNR as well as DC‑SIGN from Pigtailed Macaque and Rhesus Macaque (10).
Source
Monoclonal Mouse IgG2a Clone # DC28
Immunogen
E. coli-derived recombinant human DC-SIGN
Extracellular domain
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
Human DC-SIGN transfected 3T3 mouse embryonic fibroblast cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: DC-SIGN+DC-SIGNR

DC-SIGN (Dendritic Cell- Specific ICAM-3 Grabbing Non-Integrin) has been shown to play an important role in regulating dendritic cell (DC) and T cell interactions, including antigen presentation to T cells and enhancement of transinfection of CD4+ T cells by HIV-1 (1, 2). Efforts to identify additional type II membrane proteins resulted in the isolation of a molecule related in sequence to DC-SIGN known as DC-SIGNR (DC-SIGN Related) (3, 4). DC-SIGNR shares 73 - 80% amino acid homology with DC-SIGN and is located on human chromosome 19p13.3. Its structure is similar to DC-SIGN and therefore binds mannose residues in a calcium dependent fashion, including ICAM-3 and HIV-1 gp120 (5). DC-SIGNR, also known as L-SIGN (Liver/Lymph node-Specific ICAM-3-Grabbing Non-integrin) and DC‑SIGNR, is polymorphic since allelic variations of the exon 4 encoded sequence have been isolated (5). This is further supported by a study demonstrating the ability to isolate a large repertoire of DC-SIGNR transcripts largely the result of alternative splicing of the 7 coding exons (6). L-SIGN/DC-SIGNR is primarily transcribed in the liver and lymph nodes but not in monocyte derived DC (5). Expression of L-SIGN/DC-SIGNR is restricted to endothelial cells derived from liver sinusoids, lymph nodes sinuses and capillaries (7) although variable expression in placenta and some monocytic cell lines has also been reported, including both membrane and soluble isoforms of the protein (6). Expression of DC-SIGN is induced during the in-vitro generation of DC from either monocytes or bone marrow progenitors, with maximal surface expression at day 7 of culture (1). Immature DC in the skin and mature DC in the tonsil have been demonstrated to express DC‑SIGN (8). Analysis of various tissues and cell lines suggests that DC-SIGN expression is restricted to DC (1) although a more recent report finds evidence of expression in placenta, resting monocytes and monocytic cell lines (6). This discrepancy may be partially related to the multiple isoforms of DC-SIGN transcripts, including both membrane and soluble forms, as well as exon splice variants reported in the latter study (6). A detailed description of the additional properties of this monoclonal antibody may be found in references 9 and 10.

References
  1. Geijtenbeek, T.B.H. et al. (2000) Cell 100:575.
  2. Geijtenbeek, T.B.H. et al. (2000) Cell 100:587.
  3. Yokoyama-Kobayashi, M.T. et al. (1999) Gene 228:161.
  4. Soilleux, E.J. et al. (2000) J. Immunol. 165:2937.
  5. Bashirova, A.A. et al. (2001) J. Exp. Med. 193:671.
  6. Mummidi, S. et al. (2001) J. Biol. Chem., 2001 May 3 [epub ahead of print].
  7. Pohlman, S. et al. (2001) Proc. Natl. Acad. Sci. USA 98:2670.
  8. Geijtenbeek, T.B.H. et al. (2000) Nature Immunol. 1:353.
  9. Wu, L. et al. (2002) J. Virol. 76:5905.
  10. Baribaud, F. et al. (2002) J. Virol. 76:9135.
Long Name
Dendritic Cell-specific ICAM-3-grabbing Non-integrin
Entrez Gene IDs
30835 (Human)
Alternate Names
DCSIGN+DCSIGNR; DC-SIGN+DC-SIGNR

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