Key Product Details

Species Reactivity

Human

Applications

ELISA Capture (Matched Antibody Pair)

Label

Alexa Fluor Plus 405 (Excitation = 404 nm, Emission = 455 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 560716
View all DMP-1 Primary Antibodies »

Product Specifications

Specificity

Detects human DMP-1 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant mouse DMP-1, recombinant human (rh) CD44, and rhComplement Factor H is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human DMP‑1 Alexa Fluor™ Plus 405‑conjugated Antibody

Application
Recommended Usage

ELISA Capture (Matched Antibody Pair)

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: DMP-1

Dentin matrix protein 1 (DMP-1) is a member of the SIBLING family that also includes bone sialoprotein, dentin sialophosphoprotein, MEPE, and osteopontin. These highly phosphorylated integrin-binding proteins are rich in acidic amino acids and function in the formation of calcified bone and tooth matrix (1, 2). The phosphate content, spacing of acidic residues, and calcium-dependent dimerization of DMP-1 contribute to its ability to sequester calcium phosphate clusters and promote hydroxyapatite (HA) crystal formation (3-5). Rodent DMP-1 is cleaved by BMP-1 family proteases at a single site which is conserved in human, generating a 37 kDa N-terminal and a 57 kDa C-terminal fragment (6). The N-terminal fragment in rat carries chondroitin sulfate (7). The C-terminal fragment alone can nucleate HA crystals, while crystal growth into a needle-like morphology is inhibited by the N-terminal fragment (3, 4). Crystal maturation is dependent on the presence of type I collagen (4). DMP-1 is required for odontoblast differentiation as well as dentin formation (8). Nonphosphorylated DMP-1 is targeted to the nucleus, where it activates the transcription of odontoblast and osteoblast specific genes (9, 10). Early in osteoblast maturation, nuclear DMP-1 is extensively phosphorylated by casein kinase II, triggering its secretion (9). DMP-1 mutations in humans are associated with hypophosphatemia and FGF23 overexpression (11, 12). DMP-1 induces the activation of proMMP-9 and displaces mature MMP-9 from TIMP1 (13). DMP-1 tethering of MMP-9 to the cell surface via CD44 and integrins alpha v beta 3 and alpha v beta 5 promotes tumor cell invasiveness in vitro (14). Full length DMP-1 circulates in human serum in a tight complex with complement factor H (13, 14). When first bound to CD44 or integrin alpha v beta 3, DMP-1 can anchor factor H to the cell surface and protect the cell from complement-mediated lysis (15). Mature human DMP-1 shares 61%-67% amino acid sequence identity with bovine, mouse, and rat DMP-1.

References

  1. Qin, C. et al. (2004) Crit. Rev. Oral Biol. Med. 15:126.
  2. Hirst, K.L. et al. (1997) Genomics 42:38.
  3. He, G. et al. (2003) Nat. Mater. 2:552.
  4. Gajjeraman, S. et al. (2007) J. Biol. Chem. 282:1193.
  5. He, G. et al. (2005) Biochemistry 44:16140.
  6. Steiglitz, B.M. et al. (2004) J. Biol. Chem. 279:980.
  7. Qin, C. et al. (2006 J. Biol. Chem. 281:8034.
  8. Lu, Y. et al. (2007) Dev. Biol. 303:191.
  9. Narayanan, K. et al. (2003) J. Biol. Chem. 278:17500.
  10. Narayanan, K. et al. (2006) J. Biol. Chem. 281:19064.
  11. Lorenz-Depiereux, B. et al. (2006) Nat. Genet. 38:1248.
  12. Feng, J.Q. et al. (2006) Nat. Genet. 38:1310.
  13. Fedarko, N.S. et al. (2004) FASEB J. 18:735.
  14. Karadag, A. et al. (2005) Cancer Res. 65:11545.
  15. Jain, A. et al. (2002) J. Biol. Chem. 277:13700.

Long Name

Dentin Matrix Protein 1

Alternate Names

DMP1

Entrez Gene IDs

1758 (Human); 13406 (Mouse); 25312 (Rat)

Gene Symbol

DMP1

UniProt

NP_001073380

Additional DMP-1 Products

Product Documents for Human DMP‑1 Alexa Fluor™ Plus 405‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human DMP‑1 Alexa Fluor™ Plus 405‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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