Human Dopamine D1R/DRD1 Alexa Fluor® 488-conjugated Antibody

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FAB8276G-100UG
R&D Systems Antibodies
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Human Dopamine D1R/DRD1 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Stains human Dopamine D1 R/DRD1 transfected cells but not irrelevant transfectants in flow cytometry.
Source
Monoclonal Mouse IgG2a Clone # 887438
Immunogen
NS0 mouse myeloma cell line transfected with human Dopamine D1 R/DRD1
Met1-Thr446
Accession # P21728
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
HEK293 human embryonic kidney cell line transfected with human Dopamine D1/DRD1 and eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Dopamine D1R/DRD1

Dopamine Receptor D1 (DRD1) is a 50 kDa member of class 1 GPCR superfamily and is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. DRD1 is associated with nicotine dependence, schizophrenia, and systolic blood pressure levels.

Long Name
Dopamine D1 Receptor
Entrez Gene IDs
1812 (Human); 13488 (Mouse); 24316 (Rat)
Alternate Names
D(1A) dopamine receptor; D1DR; DADR; Dopamine D1 R; Dopamine D1 receptor; Dopamine D1R; dopamine receptor D1; DRD1; DRD1A

Product Datasheets

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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Reviews for Human Dopamine D1R/DRD1 Alexa Fluor® 488-conjugated Antibody

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Human Dopamine D1R/DRD1 Alexa Fluor® 488-conjugated Antibody
By Anonymous on 12/17/2020
Application: Flow Cytometry Sample Tested: Whole blood Species: Human

Whole blood was diluted with PBS, overlaid atop Ficoll, and centrifuged to separate the PBMC layer. The PBMC layer was washed with PBS. Cells concentration was adjusted to 10^4 cells per uL. 10^6 of cells were aliquoted into centrifuge tubes for staining.

1uL DRD1 antibody was added to cell suspension. Cells were incubated on ice for 30 mins protected from light. Following incubation, cells were washed twice with PBS and then fixed at room temperature for 30 mins protected from light. Cells were washed twice with permeabilization buffer and resuspended in 500 uL PBS for flow cytometry acquisition.

Black graph depicts unstained PBMCs. Red graph depicts DRD1 stained PBMCs.