Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 887438
View all Dopamine D1R/DRD1 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human Dopamine D1 R/DRD1
Met1-Thr446
Accession # P21728

Specificity

Stains human Dopamine D1 R/DRD1 transfected cells but not irrelevant transfectants in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human Dopamine D1R/DRD1 Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human Dopamine D1/DRD1 and eGFP

Reviewed Applications

Read 1 review rated 4 using FAB8276G in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Dopamine D1R/DRD1

Dopamine Receptor D1 (DRD1) is a 50 kDa member of class 1 GPCR superfamily and is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. DRD1 is associated with nicotine dependence, schizophrenia, and systolic blood pressure levels.

Long Name

Dopamine D1 Receptor

Alternate Names

DADR, Dopamine D1 R, DRD1

Entrez Gene IDs

1812 (Human); 13488 (Mouse); 24316 (Rat)

Gene Symbol

DRD1

UniProt

P21728

Additional Dopamine D1R/DRD1 Products

Product Documents for Human Dopamine D1R/DRD1 Alexa Fluor® 488-conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human Dopamine D1R/DRD1 Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

Customer Reviews for Human Dopamine D1R/DRD1 Alexa Fluor® 488-conjugated Antibody (1)

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  • Human Dopamine D1R/DRD1 Alexa Fluor® 488-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Whole blood
    Species: Human
    Verified Customer | Posted 12/17/2020
    Whole blood was diluted with PBS, overlaid atop Ficoll, and centrifuged to separate the PBMC layer. The PBMC layer was washed with PBS. Cells concentration was adjusted to 10^4 cells per uL. 10^6 of cells were aliquoted into centrifuge tubes for staining. 1uL DRD1 antibody was added to cell suspension. Cells were incubated on ice for 30 mins protected from light. Following incubation, cells were washed twice with PBS and then fixed at room temperature for 30 mins protected from light. Cells were washed twice with permeabilization buffer and resuspended in 500 uL PBS for flow cytometry acquisition. Black graph depicts unstained PBMCs. Red graph depicts DRD1 stained PBMCs.
    Human Dopamine D1R/DRD1 Alexa Fluor® 488-conjugated Antibody FAB8276G

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