Mouse IgG2A Alexa Fluor® 488-conjugated Antibody

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Detection of Mouse IgG Isotype Control by Flow Cytometry
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Product Details
Citations (4)
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Mouse IgG2A Alexa Fluor® 488-conjugated Antibody Summary

Selected for use as mouse IgG2A isotype control for flow cytometry applications.
Monoclonal Mouse IgG2A Clone # 20102
Protein A or G purified from hybridoma culture supernatant
Supplied in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)


Recommended Concentration
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 5 μL/106 cells.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Detection of Mouse IgG Control by Flow Cytometry BG01V human embryonic stem cells were stained with Mouse Anti-Human Podocalyxin Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB1658G, filled histogram) or Mouse IgG Alexa Fluor® 488-conjugated Isotype Control Antibody (Catalog # IC003G, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2A Fc
Alternate Names

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Citations for Mouse IgG2A Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Circulating ACE2-expressing extracellular vesicles block broad strains of SARS-CoV-2
    Authors: L El-Shennaw, AD Hoffmann, NK Dashzeveg, KM McAndrews, PJ Mehl, D Cornish, Z Yu, VL Tokars, V Nicolaescu, A Tomatsidou, C Mao, CJ Felicelli, CF Tsai, C Ostiguin, Y Jia, L Li, K Furlong, J Wysocki, X Luo, CF Ruivo, D Batlle, TJ Hope, Y Shen, YK Chae, H Zhang, VS LeBleu, T Shi, S Swaminatha, Y Luo, D Missiakas, GC Randall, AR Demonbreun, MG Ison, R Kalluri, D Fang, H Liu
    Nature Communications, 2022;13(1):405.
    Species: Human
    Sample Types: Extracellular Vesicles
    Applications: Electron Microscopy Control
  2. Application of small molecule FPR1 antagonists in the treatment of cancers
    Authors: DS Ahmet, HA Basheer, A Salem, D Lu, A Aghamohamm, P Weyerhäuse, A Bordiga, J Almeniawi, S Rashid, PA Cooper, SD Shnyder, V Vinader, K Afarinkia
    Sci Rep, 2020;10(1):17249.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Acetyl-CoA Carboxylase inhibition improves multiple dimensions of NASH pathogenesis in model systems
    Authors: TT Ross, C Crowley, KL Kelly, A Rinaldi, DA Beebe, MP Lech, RV Martinez, S Carvajal-G, M Boucher, D Hirenallur, J Morin, AC Opsahl, SR Vargas, KK Bence, JA Pfefferkor, WP Esler
    Cell Mol Gastroenterol Hepatol, 2020;0(0):.
    Species: Rat
    Sample Types: Whole Tissues
    Applications: IHC
  4. Targeting of cadherin-11 decreases skin fibrosis in the tight skin-1 mouse model
    Authors: M Pedroza, RL Welschhans, SK Agarwal
    PLoS ONE, 2017;12(11):e0187109.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P


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