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Human DSPG3 Alexa Fluor® 532-conjugated Antibody

Catalog #: AF3110X Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3110X-100UG

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DSPG3 Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human DSPG3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross-reactivity with recombinant mouse DSPG3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human DSPG3
Met1-Val322
Accession # Q99645
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: DSPG3

DSPG3, also known as proteoglycan-Lb and Epiphycan, is a 95 - 100 kDa member of the class III subfamily within the SLRP (small leucine-rich proteoglycan) family. The secreted 303 aa mature segment contains multiple sites for O-linked glycanation, and seven leucine-rich repeats, flanked by N- and C-terminal cysteine-rich regions. Human DSPG3 shares 96%, 95%, 85% and 84% aa sequence identity with canine, bovine, rat and mouse DSPG3, respectively. DSPG3 is principally a component of cartilage.

Long Name
Dermatan Sulfate Proteoglycan 3
Entrez Gene IDs
1833 (Human); 13516 (Mouse)
Alternate Names
Dermatan sulfate proteoglycan 3dermatan sulphate proteoglycan 3; DSPG3; DSPG3proteoglycan-lb; Epiphycan; EPYC; PGLB; PG-Lb; Proteoglycan-Lb; SLRR3B; SLRR3Bepiphycan proteoglycan; Small chondroitin/dermatan sulfate proteoglycan

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