Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Bioassay

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # BBIG-E6 (13D5)
View all E-Selectin (CD62E)/P-Selectin (CD62P) Primary Antibodies »

Product Specifications

Immunogen

Activated HUVEC human umbilical vein endothelial cells

Specificity

Detects human E‑Selectin/P‑Selectin (CD62E/P). Binds to COS cells transfected with human E-Selectin or human P-Selectin. It does not bind to CHO cells transfected with human ICAM-1, L-Selectin, PECAM-1 or VCAM-1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human E‑Selectin/P‑Selectin (CD62E/P) APC‑conjugated Antibody

Detection of E-Selectin/P-Selectin (CD62E/P) antibody in HUVEC Human Cells antibody by Flow Cytometry.

Detection of E‑Selectin/P‑Selectin (CD62E/P) in HUVEC Human Cells by Flow Cytometry.

HUVEC human umbilical vein endothelial cells treated with Recombinant Human TNF-a (Catalog # 210-TA) were stained with Mouse Anti-Human E-Selectin/P-Selectin (CD62E/P) APC-conjugated Monoclonal Antibody (Catalog # FAB6169A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human E‑Selectin/P‑Selectin (CD62E/P) APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HUVEC human umbilical vein endothelial cells treated with Recombinant Human TNF‑ alpha (Catalog # 210-TA)

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: E-Selectin (CD62E)/P-Selectin (CD62P)

E-Selectin (CD62E) and P-Selectin (CD62P) are related (44% amino acid sequence identity) adhesion molecules containing multiple short complement-like repeats and an N-terminal C-type lectin domain in their extracellular regions. E-Selectin is expressed on activated vascular endothelial cells and P-Selectin occurs on activated platelets and endothelial cells.

Alternate Names

Eselectin, PSelectin

Additional E-Selectin (CD62E)/P-Selectin (CD62P) Products

Product Documents for Human E‑Selectin/P‑Selectin (CD62E/P) APC‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human E‑Selectin/P‑Selectin (CD62E/P) APC‑conjugated Antibody

For research use only

Citations for Human E‑Selectin/P‑Selectin (CD62E/P) APC‑conjugated Antibody

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