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Recombinant Human TNF-alpha Protein

R&D Systems | Catalog # 210-TA

GMP Version Available: 210-GMP
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Analyzed by SEC-MALS
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Key Product Details

  • R&D Systems E. coli-derived Recombinant Human TNF-alpha Protein (210-TA)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

P01375

Structure / Form

Homotrimer

Applications

Bioactivity
View all TNF-alpha Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human TNF-alpha protein
Val77-Leu233, with and without an N-terminal Met

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met & Val77

Predicted Molecular Mass

17 kDa

SDS-PAGE

17 kDa, reducing conditions

Activity

Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221.
The ED50 for this effect is 25-100 pg/mL.

Reviewed Applications

Read 41 reviews rated 4.8 using 210-TA in the following applications:

Scientific Data Images for Recombinant Human TNF-alpha Protein

Recombinant Human TNF‑ alpha Protein SEC-MALS.

Recombinant Human TNF-alpha (Catalog # 210-TA) has a molecular weight (MW) of 53.1 kDa as analyzed by SEC-MALS, suggesting that this protein is a homotrimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).
Recombinant Human TNF-alpha Protein Bioactivity

Recombinant Human TNF-alpha Protein Bioactivity

Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
Recombinant Human TNF-alpha Protein SDS-PAGE

Recombinant Human TNF-alpha Protein SDS-PAGE

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 210-TA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 0.1-1 mg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 210-TA/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS. *Small pack size (5 & 20 µg) is supplied as a 0.2 µm filtered solution in PBS. 
Reconstitution Reconstitute at 0.1-1 mg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (5 & 20 µg) is shipped with dry ice or equivalent. Upon receipt, store it immediately at -20 to -70 °C.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TNF-alpha

Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism (1, 2). Human TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD) (3). Within the ECD, human TNF-alpha shares 97% aa sequence identity with rhesus and 71%-92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-alpha. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells (1, 2). TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface (4). Cell surface TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I (2, 5). Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa soluble trimer of the TNF-alpha extracellular domain (6-8). TNF-alpha binds the ubiquitous 55-60 kDa TNF RI (9, 10) and the hematopoietic cell-restricted 80 kDa TNF RII (11, 12), both of which are also expressed as homotrimers (1, 2, 13). Both type I and type II receptors bind TNF-alpha with comparable affinity (14), although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha (15).

References

  1. Zelova, H. and J. Hosek (2013) Inflamm. Res. 62:641.
  2. Juhasz, K. et al. (2013) Expert Rev. Clin. Immunol. 9:335.
  3. Pennica, D. et al. (1984) Nature 312:724.
  4. Tang, P. et al. (1996) Biochemistry 35:8216.
  5. Perez, C. et al. (1990) Cell 63:251.
  6. Black, R.A. et al. (1997) Nature 385:729.
  7. Moss, M.L. et al. (1997) Nature 385:733.
  8. Gearing, A.J.H. et al. (1994) Nature 370:555.
  9. Schall, T.J. et al. (1990) Cell 61:361.
  10. Loetscher, H. et al. (1990) Cell 61:351.
  11. Dembic, Z. et al. (1990) Cytokine 2:231.
  12. Smith, C.A. et al. (1990) Science 248:1019.
  13. Loetscher, H. et al. (1991) J. Biol. Chem. 266:18324.
  14. Pinckard, J.K. et al. (1997) J. Biol. Chem. 272:10784.
  15. Engelmann, H. et al. (1990) J. Biol. Chem. 265:1531.

Long Name

Tumor Necrosis Factor alpha

Alternate Names

Cachetin, DIF, TNF, TNF-A, TNFA, TNFalpha, TNFG1F, TNFSF1A, TNFSF2

Entrez Gene IDs

7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 102139631 (Cynomolgus Monkey); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)

Gene Symbol

TNF

UniProt

P01375

Additional TNF-alpha Products

Product Documents for Recombinant Human TNF-alpha Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 210-TA (with carrier)
Download SDS 210-TA/CF (carrier free)

Product Specific Notices for Recombinant Human TNF-alpha Protein

For research use only

Citations for Recombinant Human TNF-alpha Protein

Customer Reviews for Recombinant Human TNF-alpha Protein (41)

4.8 out of 5
41 Customer Ratings
5 Stars
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4 Stars
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Showing  1 - 5 of 41 reviews Showing All
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  • Recombinant Human TNF-alpha Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 06/03/2025
    The cell proliferation assay showed a clear increase in cell growth with higher mitogen concentrations, confirming the assay works well.
    Recombinant Human TNF-alpha Protein 210-TA
  • Recombinant Human TNF-alpha Protein
    Name: Lana Jagannathan
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 11/01/2023
    Dendritic cell CD40 cell surface expression increases with the addition of GM-CSF, IL-4, TNF-alpha, IL-6, IL-1B, and LPS.
    Recombinant Human TNF-alpha Protein 210-TA
  • Recombinant Human TNF-alpha Protein
    Name: Russ Tas
    Application: Apoptosis assay
    Verified Customer | Posted 08/14/2023
  • Recombinant Human TNF-alpha Protein
    Name: Russ Tas
    Application: Cell Proliferation
    Verified Customer | Posted 08/07/2023
  • Recombinant Human TNF-alpha Protein
    Name: Andres Ocampo Carrillo
    Application: In vivo study
    Verified Customer | Posted 02/24/2023
    Human TNF-a was used to induce pro-inflammatory responses in an in vivo model of arthritis using male C57BL/6J mice. rhTNF-a was able to increase accumulation of immune cells in the pannus area, and increased bone loss in the model
    Recombinant Human TNF-alpha Protein 210-TA
  • Recombinant Human TNF-alpha Protein
    Name: Martin Torres
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 10/01/2021
    Recombinant Human TNF-alpha Protein 210-TA
  • Recombinant Human TNF-alpha Protein
    Name: Anonymous
    Application: Assay validation
    Verified Customer | Posted 02/09/2021
  • Recombinant Human TNF-alpha Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 01/15/2021
  • Recombinant Human TNF-alpha Protein
    Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 11/30/2020
  • Recombinant Human TNF-alpha Protein
    Name: Anonymous
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 11/20/2020
  • Recombinant Human TNF-alpha Protein
    Name: raghavendra vadla
    Application: SDS-PAGE Control
    Verified Customer | Posted 07/03/2020
    Recombinant Human TNF-alpha Protein 210-TA
  • Recombinant Human TNF-alpha Protein
    Name: Anonymous
    Application: Slice electrophysiology
    Verified Customer | Posted 05/22/2020
    300 micron thick slices were preincubated in TNF-alpha (100 ng/ml) for 1 h before whole-cell recordings were performed from female mice. TNF alpha reduced excitability of neurons compared to control.
    Recombinant Human TNF-alpha Protein 210-TA
  • Recombinant Human TNF-alpha Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 05/01/2020
    Recombinant Human TNF-alpha Protein 210-TA
  • Recombinant Human TNF-alpha Protein
    Name: Youwen Zhang
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 04/17/2020
  • Recombinant Human TNF-alpha Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 01/14/2020
  • Recombinant Human TNF-alpha Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 01/10/2020
  • Recombinant Human TNF-alpha Protein
    Name: Anonymous
    Application: Cytotoxicity Assay
    Verified Customer | Posted 05/15/2019
    Recombinant Human TNF-alpha Protein 210-TA
  • Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 04/30/2019
  • Recombinant Human TNF-alpha Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 02/04/2019
  • Recombinant Human TNF-alpha Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 01/29/2019
  • Recombinant Human TNF-alpha Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 09/10/2018
  • Recombinant Human TNF-alpha Protein
    Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 08/06/2018
  • Recombinant Human TNF-alpha Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 05/09/2018
  • Name: Balaji Mahender
    Application: Immunoassay Standard
    Verified Customer | Posted 01/29/2018
  • Recombinant Human TNF-alpha Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 01/08/2018
    We tried it for induction of NF-KB detected with luciferase and immunoblot
    Recombinant Human TNF-alpha Protein 210-TA
  • Recombinant Human TNF-alpha Protein
    Name: Anonymous
    Application: In vivo study
    Verified Customer | Posted 10/30/2017
  • Name: Billie Caison
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 07/17/2017
    Recombinant Human TNF-alpha Protein 210-TA
  • Name: Boubacar Barry
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 06/01/2017
  • Name: Anonymous
    Application: Cell migration/motility
    Verified Customer | Posted 05/23/2017
  • Recombinant Human TNF-alpha Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 05/16/2017
    We used TNF-a to stimulation macrophages and myofibroblast
  • Name: Sitara Sankar
    Verified Customer | Posted 05/15/2017
  • Recombinant Human TNF-alpha Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 12/19/2016
  • Recombinant Human TNF-alpha Protein
    Name: Veronica Fregoso
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 08/01/2016
    Rat primary embryonic cortical culture stimulated with TNFa (10, 50, 100 ng/ml), PBS+BSA (negative stimulation control), or Okadaic Acid (OA; positive phosphorylation control) for 30 minutes. Protein isolated for western blot analysis of phospho-Akt.
    Recombinant Human TNF-alpha Protein 210-TA
  • Recombinant Human TNF-alpha Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 07/29/2016
    Cultured endothelial cells were pre-treated (or not) with 1mg/mL HDL, then treated for 6 hours with 10ng/mL TNFa. TNFa treatment caused a massive upregulation of ICAM1 and E-Selectin versus control cells (untreated); HDL pre-treatment partially reversed this response.
    Recombinant Human TNF-alpha Protein 210-TA
  • Recombinant Human TNF-alpha Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 07/21/2016
  • Recombinant Human TNF-alpha Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 07/21/2016
  • Recombinant Human TNF-alpha Protein
    Name: deborah webb
    Application: Immunoassay Standard
    Verified Customer | Posted 05/16/2016
  • Recombinant Human TNF-alpha Protein
    Name: jinyan yu
    Application: SDS-PAGE Control
    Verified Customer | Posted 05/12/2016
    Recombinant Human TNF-alpha Protein 210-TA
  • Recombinant Human TNF-alpha Protein
    Name: WENJIE ZHOU
    Application: Cell Adhesion
    Verified Customer | Posted 02/12/2016
    Recombinant Human TNF-alpha Protein 210-TA
  • Recombinant Human TNF-alpha Protein
    Name: Yang Luo
    Application: Cell Adhesion
    Verified Customer | Posted 01/29/2016
    Recombinant Human TNF-alpha Protein 210-TA
  • Recombinant Human TNF-alpha Protein
    Name: Mengshi Ma
    Application: Stimulation of human epithelial cells
    Verified Customer | Posted 01/13/2016
    Use TNF-alpha to stimulate human epithelial cell, the production of inflammation factor is stable and strongly evaluated by western blot and ELISA.

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