Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Rat, Porcine, Insect - Aedes albopictus (Asian tiger mosquito)

Applications

Validated:

Adhesion Blockade, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, CyTOF-ready

Cited:

Immunohistochemistry, Neutralization, Flow Cytometry, Immunocytochemistry, ELISA Capture

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # BBIG-E6 (13D5)
View all E-Selectin (CD62E)/P-Selectin (CD62P) Primary Antibodies »

Product Specifications

Immunogen

Activated HUVEC human umbilical vein endothelial cells

Specificity

Detects human E‑Selectin/P‑Selectin (CD62E/P). Binds to COS cells transfected with human E-Selectin or human P-Selectin. It does not bind to CHO cells transfected with human ICAM-1, L-Selectin, PECAM-1 or VCAM-1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human E‑Selectin/P‑Selectin (CD62E/P) Antibody

E-Selectin (CD62E)/P-Selectin (CD62P) antibody in HUVEC Human Cells by Immunocytochemistry (ICC).

E-Selectin (CD62E)/P-Selectin (CD62P) in HUVEC Human Cells.

E-Selectin (CD62E)/P-Selectin (CD62P) was detected in immersion fixed HUVEC human umbilical vein endothelial cells stimulated with TNF-alpha (Catalog # 210-TA-010) using Mouse Anti-Human E-Selectin (CD62E)/P-Selectin (CD62P) Monoclonal Antibody (Catalog # BBA1) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human E‑Selectin/P‑Selectin (CD62E/P) Antibody

Application
Recommended Usage

Adhesion Blockade

The adhesion of U937 human histiocytic lymphoma cells (5 x 104 cells/well) to immobilized Recombinant Human P-Selectin/CD62P (Catalog # ADP3, 10 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 10 µg/mL of the antibody.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: HUVEC human umbilical vein endothelial cells treated with Recombinant Human TNF‑ alpha (Catalog # 210-TA)

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells activated with recombinant human TNF-alpha (Catalog # 210-TA-010)

Immunoprecipitation

Pigott, R. et al. (1991) J. Immunol. 147:130.

Reviewed Applications

Read 4 reviews rated 3.3 using BBA1 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: E-Selectin (CD62E)/P-Selectin (CD62P)

E-Selectin and P-Selectin are related (44% amino acid sequence identity) adhesion molecules containing multiple short complement-like repeats and an N-terminal C‑type lectin domain in their extracellular regions. E-Selectin is expressed on activated vascular endothelial cells and P-Selectin occurs on activated platelets and endothelial cells.

Alternate Names

Eselectin, PSelectin

Additional E-Selectin (CD62E)/P-Selectin (CD62P) Products

Product Documents for Human E‑Selectin/P‑Selectin (CD62E/P) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human E‑Selectin/P‑Selectin (CD62E/P) Antibody

For research use only

Citations for Human E‑Selectin/P‑Selectin (CD62E/P) Antibody

Customer Reviews for Human E‑Selectin/P‑Selectin (CD62E/P) Antibody (4)

3.3 out of 5
4 Customer Ratings
5 Stars
25%
4 Stars
25%
3 Stars
25%
2 Stars
0%
1 Stars
25%

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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Human E-Selectin/P-Selectin (CD62E/P) Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Сolon adenocarcinoma tissue
    Species: Human
    Verified Customer | Posted 10/31/2021
    Human E‑Selectin/P‑Selectin (CD62E/P) Antibody BBA1
  • Human E-Selectin/P-Selectin (CD62E/P) Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HUVEC human umbilical vein endothelial cells
    Species: Human
    Verified Customer | Posted 11/10/2018
    Western blot using standard conditions on LPS or tumor secretion-stimulated HUVEC cells. No specific bands present.
    Human E‑Selectin/P‑Selectin (CD62E/P) Antibody BBA1
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.
  • Human E-Selectin/P-Selectin (CD62E/P) Antibody
    Name: Ruchi Gupta
    Application: Western Blot
    Sample Tested: Human cells
    Species: Human
    Verified Customer | Posted 07/09/2018
    Human E‑Selectin/P‑Selectin (CD62E/P) Antibody BBA1
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.
  • Human E-Selectin/P-Selectin (CD62E/P) Antibody
    Name: Ruchi Gupta
    Application: Immunohistochemistry
    Sample Tested: Human cells
    Species: Human
    Verified Customer | Posted 08/18/2017
    Human E‑Selectin/P‑Selectin (CD62E/P) Antibody BBA1

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Protocols

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