Human E-Selectin/P-Selectin (CD62E/P) Antibody

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BBA1

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E-Selectin (CD62E)/P-Selectin (CD62P) in HUVEC Human Cells.
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Citations (12)
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Reviews (4)

Human E-Selectin/P-Selectin (CD62E/P) Antibody Summary

Species Reactivity
Human
Specificity
Detects human E‑Selectin/P‑Selectin (CD62E/P). Binds to COS cells transfected with human E-Selectin or human P-Selectin. It does not bind to CHO cells transfected with human ICAM-1, L-Selectin, PECAM-1 or VCAM-1.
Source
Monoclonal Mouse IgG1 Clone # BBIG-E6 (13D5)
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Activated HUVEC human umbilical vein endothelial cells
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
HUVEC human umbilical vein endothelial cells treated with Recombinant Human TNF‑ alpha (Catalog # 210-TA)
Immunoprecipitation
Pigott, R. et al. (1991) J. Immunol. 147:130.
 
Adhesion Blockade
The adhesion of U937 human histiocytic lymphoma cells (5 x 104 cells/well) to immobilized Recombinant Human P-Selectin/CD62P (Catalog # ADP3, 10 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 10 µg/mL of the antibody.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry E-Selectin (CD62E)/P-Selectin (CD62P) antibody in HUVEC Human Cells by Immunocytochemistry (ICC). View Larger

E-Selectin (CD62E)/P-Selectin (CD62P) in HUVEC Human Cells. E-Selectin (CD62E)/P-Selectin (CD62P) was detected in immersion fixed HUVEC human umbilical vein endothelial cells stimulated with TNF-alpha (Catalog # 210-TA-010) using Mouse Anti-Human E-Selectin (CD62E)/P-Selectin (CD62P) Monoclonal Antibody (Catalog # BBA1) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: E-Selectin (CD62E)/P-Selectin (CD62P)

E-Selectin and P-Selectin are related (44% amino acid sequence identity) adhesion molecules containing multiple short complement-like repeats and an N-terminal C‑type lectin domain in their extracellular regions. E-Selectin is expressed on activated vascular endothelial cells and P-Selectin occurs on activated platelets and endothelial cells.

Alternate Names
CD62E; ELAM; ELAM1; ESEL; Eselectin; E-Selectin/P-Selectin; LECAM2; PSelectin

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Citations for Human E-Selectin/P-Selectin (CD62E/P) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Sulfonated Amphiphilic Poly(alpha)glutamate Amine-A Potential siRNA Nanocarrier for the Treatment of Both Chemo-Sensitive and Chemo-Resistant Glioblastoma Tumors
    Authors: A Krivitsky, S Pozzi, E Yeini, S Israeli Da, T Zur, S Golan, V Krivitsky, N Albeck, E Pisarevsky, P Ofek, A Madi, R Satchi-Fai
    Pharmaceutics, 2021-12-20;13(12):.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC
  2. Microengineered perfusable 3D-bioprinted glioblastoma model for in vivo mimicry of tumor microenvironment
    Authors: L Neufeld, E Yeini, N Reisman, Y Shtilerman, D Ben-Shusha, S Pozzi, A Madi, G Tiram, A Eldar-Booc, S Ferber, R Grossman, Z Ram, R Satchi-Fai
    Science Advances, 2021-08-18;7(34):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, IHC
  3. P-selectin axis plays a key role in microglia immunophenotype and glioblastoma progression
    Authors: E Yeini, P Ofek, S Pozzi, N Albeck, D Ben-Shusha, G Tiram, S Golan, R Kleiner, R Sheinin, S Israeli Da, S Reich-Zeli, R Grossman, Z Ram, H Brem, TM Hyde, P Magod, D Friedmann-, A Madi, R Satchi-Fai
    Nature Communications, 2021-03-26;12(1):1912.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Zika Virus Infection Promotes Local Inflammation, Cell Adhesion Molecule Upregulation, and Leukocyte Recruitment at the Blood-Brain Barrier
    Authors: M Clé, C Desmetz, J Barthelemy, MF Martin, O Constant, G Maarifi, V Foulongne, K Bolloré, Y Glasson, F De Bock, M Blaquiere, L Dehouck, N Pirot, E Tuaillon, S Nisole, F Najioullah, P Van de Per, A Cabié, N Marchi, F Gosselet, Y Simonin, S Salinas
    MBio, 2020-08-04;11(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Obesity-Altered Adipose Stem Cells Promote Radiation Resistance of Estrogen Receptor Positive Breast Cancer through Paracrine Signaling
    Authors: RA Sabol, VA Villela, A Denys, BT Freeman, AB Hartono, RM Wise, MAA Harrison, MB Sandler, F Hossain, L Miele, BA Bunnell
    Int J Mol Sci, 2020-04-15;21(8):.
  6. Haptoglobin and hemopexin inhibit vaso-occlusion and inflammation in murine sickle cell disease: Role of heme oxygenase-1 induction
    Authors: JD Belcher, C Chen, J Nguyen, F Abdulla, P Zhang, H Nguyen, P Nguyen, T Killeen, SM Miescher, N Brinkman, KA Nath, CJ Steer, GM Vercellott
    PLoS ONE, 2018-04-25;13(4):e0196455.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. Self-medication by orang-utans (Pongo pygmaeus) using bioactive properties of Dracaena cantleyi
    Authors: HC Morrogh-Be, I Foitová, Z Yeen, P Wilkin, R de Martin, L Rárová, K Doležal, W Nurcahyo, M Olšanský
    Sci Rep, 2017-11-30;7(1):16653.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Prenylated Polyphenols from Clusiaceae and Calophyllaceae with Immunomodulatory Activity on Endothelial Cells
    PLoS ONE, 2016-12-01;11(12):e0167361.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. SDF-1?-induced dual pairs of E-selectin/ligand mediate endothelial progenitor cell homing to critical ischemia
    Sci Rep, 2016-10-07;6(0):34416.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. West Nile virus-induced cell adhesion molecules on human brain microvascular endothelial cells regulate leukocyte adhesion and modulate permeability of the in vitro blood-brain barrier model.
    Authors: Roe, Kelsey, Orillo, Beverly, Verma, Saguna
    PLoS ONE, 2014-07-18;9(7):e102598.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Functional diversity of human vaginal APC subsets in directing T-cell responses.
    Authors: Duluc, D, Gannevat, J, Anguiano, E, Zurawski, S, Carley, M, Boreham, M, Stecher, J, Dullaers, M, Banchereau, J, Oh, S
    Mucosal Immunol, 2012-11-07;6(3):626-38.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Flow Cytometry
  12. Activation of primary porcine endothelial cells induces release of porcine endogenous retroviruses.
    Authors: Cunningham DA, Dos Santos Cruz GJ, Fernandez-Suarez XM, Whittam AJ, Herring C, Copeman L, Richards A, Langford GA
    Transplantation, 2004-04-15;77(7):1071-9.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human E-Selectin/P-Selectin (CD62E/P) Antibody

Average Rating: 3.3 (Based on 4 Reviews)

5 Star
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Human E-Selectin/P-Selectin (CD62E/P) Antibody
By Anonymous on 10/31/2021
Application: IHC Sample Tested: Сolon adenocarcinoma tissue Species: Human

Human E-Selectin/P-Selectin (CD62E/P) Antibody
By Anonymous on 11/10/2018
Application: WB Sample Tested: HUVEC human umbilical vein endothelial cells Species: Human

Western blot using standard conditions on LPS or tumor secretion-stimulated HUVEC cells. No specific bands present.
***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***


Human E-Selectin/P-Selectin (CD62E/P) Antibody
By Ruchi Gupta on 07/09/2018
Application: WB Sample Tested: Human cells Species: Human

***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***


Human E-Selectin/P-Selectin (CD62E/P) Antibody
By Ruchi Gupta on 08/18/2017
Application: IHC Sample Tested: Human cells Species: Human