Human E-Selectin/P-Selectin (CD62E/P) Antibody
Human E-Selectin/P-Selectin (CD62E/P) Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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E-Selectin (CD62E)/P-Selectin (CD62P) in HUVEC Human Cells. E-Selectin (CD62E)/P-Selectin (CD62P) was detected in immersion fixed HUVEC human umbilical vein endothelial cells stimulated with TNF-alpha (Catalog # 210-TA-010) using Mouse Anti-Human E-Selectin (CD62E)/P-Selectin (CD62P) Monoclonal Antibody (Catalog # BBA1) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: E-Selectin (CD62E)/P-Selectin (CD62P)
E-Selectin and P-Selectin are related (44% amino acid sequence identity) adhesion molecules containing multiple short complement-like repeats and an N-terminal C‑type lectin domain in their extracellular regions. E-Selectin is expressed on activated vascular endothelial cells and P-Selectin occurs on activated platelets and endothelial cells.
Product Datasheets
Citations for Human E-Selectin/P-Selectin (CD62E/P) Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
12
Citations: Showing 1 - 10
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P-selectin axis plays a key role in microglia immunophenotype and glioblastoma progression
Authors: E Yeini, P Ofek, S Pozzi, N Albeck, D Ben-Shusha, G Tiram, S Golan, R Kleiner, R Sheinin, S Israeli Da, S Reich-Zeli, R Grossman, Z Ram, H Brem, TM Hyde, P Magod, D Friedmann-, A Madi, R Satchi-Fai
Nature Communications, 2021;12(1):1912.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Microengineered perfusable 3D-bioprinted glioblastoma model for in vivo mimicry of tumor microenvironment
Authors: L Neufeld, E Yeini, N Reisman, Y Shtilerman, D Ben-Shusha, S Pozzi, A Madi, G Tiram, A Eldar-Booc, S Ferber, R Grossman, Z Ram, R Satchi-Fai
Science Advances, 2021;7(34):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Sulfonated Amphiphilic Poly(alpha)glutamate Amine-A Potential siRNA Nanocarrier for the Treatment of Both Chemo-Sensitive and Chemo-Resistant Glioblastoma Tumors
Authors: A Krivitsky, S Pozzi, E Yeini, S Israeli Da, T Zur, S Golan, V Krivitsky, N Albeck, E Pisarevsky, P Ofek, A Madi, R Satchi-Fai
Pharmaceutics, 2021;13(12):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Obesity-Altered Adipose Stem Cells Promote Radiation Resistance of Estrogen Receptor Positive Breast Cancer through Paracrine Signaling
Authors: RA Sabol, VA Villela, A Denys, BT Freeman, AB Hartono, RM Wise, MAA Harrison, MB Sandler, F Hossain, L Miele, BA Bunnell
Int J Mol Sci, 2020;21(8):.
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Zika Virus Infection Promotes Local Inflammation, Cell Adhesion Molecule Upregulation, and Leukocyte Recruitment at the Blood-Brain Barrier
Authors: M Clé, C Desmetz, J Barthelemy, MF Martin, O Constant, G Maarifi, V Foulongne, K Bolloré, Y Glasson, F De Bock, M Blaquiere, L Dehouck, N Pirot, E Tuaillon, S Nisole, F Najioullah, P Van de Per, A Cabié, N Marchi, F Gosselet, Y Simonin, S Salinas
MBio, 2020;11(4):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Haptoglobin and hemopexin inhibit vaso-occlusion and inflammation in murine sickle cell disease: Role of heme oxygenase-1 induction
Authors: JD Belcher, C Chen, J Nguyen, F Abdulla, P Zhang, H Nguyen, P Nguyen, T Killeen, SM Miescher, N Brinkman, KA Nath, CJ Steer, GM Vercellott
PLoS ONE, 2018;13(4):e0196455.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Self-medication by orang-utans (Pongo pygmaeus) using bioactive properties of Dracaena cantleyi
Authors: HC Morrogh-Be, I Foitová, Z Yeen, P Wilkin, R de Martin, L Rárová, K Doležal, W Nurcahyo, M Olšanský
Sci Rep, 2017;7(1):16653.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
SDF-1?-induced dual pairs of E-selectin/ligand mediate endothelial progenitor cell homing to critical ischemia
Sci Rep, 2016;6(0):34416.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Prenylated Polyphenols from Clusiaceae and Calophyllaceae with Immunomodulatory Activity on Endothelial Cells
PLoS ONE, 2016;11(12):e0167361.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
West Nile virus-induced cell adhesion molecules on human brain microvascular endothelial cells regulate leukocyte adhesion and modulate permeability of the in vitro blood-brain barrier model.
Authors: Roe, Kelsey, Orillo, Beverly, Verma, Saguna
PLoS ONE, 2014;9(7):e102598.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Functional diversity of human vaginal APC subsets in directing T-cell responses.
Authors: Duluc, D, Gannevat, J, Anguiano, E, Zurawski, S, Carley, M, Boreham, M, Stecher, J, Dullaers, M, Banchereau, J, Oh, S
Mucosal Immunol, 2013;6(3):626-38.
Species: Human
Sample Types: Tissue Homogenates
Applications: Flow Cytometry -
Activation of primary porcine endothelial cells induces release of porcine endogenous retroviruses.
Authors: Cunningham DA, Dos Santos Cruz GJ, Fernandez-Suarez XM, Whittam AJ, Herring C, Copeman L, Richards A, Langford GA
Transplantation, 2004;77(7):1071-9.
Species: Porcine
Sample Types: Whole Cells
Applications: Flow Cytometry
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Reviews for Human E-Selectin/P-Selectin (CD62E/P) Antibody
Average Rating: 3.3 (Based on 4 Reviews)
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Western blot using standard conditions on LPS or tumor secretion-stimulated HUVEC cells. No specific bands present.
***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***
***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***
