Human EBI3 APC-conjugated Antibody Summary
Accession # Q14213
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of IL‑27/IL‑35 EBI3 Subunit in KG‑1 Human Cell Line by Flow Cytometry. KG-1 human acute myelogenous leukemia cell line was stained with Mouse Anti-Human IL-27/IL-35 EBI3 Subunit APC-conjugated Monoclonal Antibody (Catalog # IC6456A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
IL-27 is a heterodimeric cytokine comprised of the IL-12 p35-related protein, p28, and the IL-12 p40-related protein, EBI3 (Epstein-Barr virus-Induced gene 3). IL-27 is expressed by monocytes, endothelial cells and dendritic cells. It binds TCCR/WSX-1 on naive CD4+ T cells and induces the expression of a functional IL-12 receptor, making these cells sensitive to IL-12-mediated Th1 cell development. Human EBI3 also associates with the p35 subunit of IL-12 to form IL-35 which is important for immunosuppressive Treg cell induction. EBI3 is 61% amino acid (aa) identical to mouse EBI3 and includes an 20 aa signal peptide and a 209 aa mature protein with two fibronectin type III domains.
Citation for Human EBI3 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
IL-35 over-expression increases apoptosis sensitivity and suppresses cell growth in human cancer cells.
Biochem Biophys Res Commun, 2013;430(1):364-9.
Sample Types: Whole Cells
Applications: Flow Cytometry
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