Human Endoglycan/PODXL2 Alexa Fluor® 750-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1524S-100UG
R&D Systems Antibodies
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Human Endoglycan/PODXL2 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Endoglycan/PODXL2 in direct ELISAs.
Source
Monoclonal Mouse IgG2a Clone # 211816
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Endoglycan/PODXL2
Gly33-Thr500
Accession # Q9NZ53
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
HUVEC human umbilical vein endothelial cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Endoglycan/PODXL2

Endoglycan, also named Podocalyxin-like 2 protein, is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology. It is expressed in hematopoietic precursors, endothelial cells, smooth muscle cells and leukocyte subpopulations. Endoglycan is a potential marker for hematopoietic progenitors. It also functions as an L-Selectin ligand and may be involved in the inhibition of stem cell differentiation and leukocyte-endothelial adhesion (1, 2).

References
  1. Sassetti, C. et al. (2000) J. Biol. Chem. 275:9001.
  2. Fieger, C.B. et al. (2003) J. Biol. Chem. 278:27390.
Entrez Gene IDs
50512 (Human); 319655 (Mouse)
Alternate Names
Endoglycan; PODLX2; podocalyxin-like 2; podocalyxin-like protein 2; PODXL2

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