Human Endoglycan/PODXL2 Antibody
Human Endoglycan/PODXL2 Antibody Summary
Accession # Q9NZ53
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Endoglycan/PODXL2 in HeLa Human Cell Line. Endoglycan/PODXL2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Endoglycan/PODXL2 Monoclonal Antibody (Catalog # MAB1524) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Endoglycan, also named Podocalyxin-like 2 protein, is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology. It is expressed in hematopoietic precursors, endothelial cells, smooth muscle cells and leukocyte subpopulations. Endoglycan is a potential marker for hematopoietic progenitors. It also functions as an L-Selectin ligand and may be involved in the inhibition of stem cell differentiation and leukocyte-endothelial adhesion (1, 2).
Citations for Human Endoglycan/PODXL2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Identification of the SHREK family of proteins as broad-spectrum host antiviral factors
Authors: D Dabbagh, S He, B Hetrick, L Chilin, A Andalibi, Y Wu
bioRxiv : the preprint server for biology, 2021-02-03;0(0):.
Sample Types: Whole Cells
Applications: ELISA Capture, ICC
Endoglycan, a member of the CD34 family of sialomucins, is a ligand for the vascular selectins.
Authors: Kerr SC, Fieger CB, Snapp KR, Rosen SD
J. Immunol., 2008-07-15;181(2):1480-90.
Sample Types: Whole Cells
Applications: Flow Cytometry
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