Endoglycan/PODXL2 in HeLa Human Cell Line.
Endoglycan/PODXL2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Endoglycan/PODXL2 Monoclonal Antibody (Catalog # MAB1524) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Endoglycan, also named Podocalyxin-like 2 protein, is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology. It is expressed in hematopoietic precursors, endothelial cells, smooth muscle cells and leukocyte subpopulations. Endoglycan is a potential marker for hematopoietic progenitors. It also functions as an L-Selectin ligand and may be involved in the inhibition of stem cell differentiation and leukocyte-endothelial adhesion (1, 2).
Sassetti, C. et al. (2000) J. Biol. Chem. 275:9001.
Fieger, C.B. et al. (2003) J. Biol. Chem. 278:27390.
Entrez Gene IDs:
50512 (Human); 319655 (Mouse)
Endoglycan; PODLX2; podocalyxin-like 2; podocalyxin-like protein 2; PODXL2
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