Detection of ENPP‑3/CD203c in Human Whole Blood Lymphocytes and Monocytes by Flow Cytometry.
Human whole blood lymphocytes and monocytes were stained with Mouse Anti-Human IL‑3 R alpha /CD123 PE‑conjugated Monoclonal Antibody (Catalog # FAB301P) and either (A) Mouse Anti-Human ENPP‑3/CD203c APC‑conjugated Monoclonal Antibody (Catalog # FAB5756A) or (B) Mouse IgG1 Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
CD203c is a 120-130 kDa type II transmembrane glycoprotein that belongs to the ectonucleotidase family, AP superfamily of enzymes. CD203c is also known as ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP-3) and it modulates purinergic signaling by hydrolysis of nucleotide di- and tri-phosphates plus sphingosylphosphorylenosine. Although CD203c is considered to be a marker for activated basophils and mast cells, it is also found constitutively or inducibly on bile duct epithelium, hepatocytes, duodenal columnar epithelium, osteoblasts, plasmacytoid dendritic cells, airway pseudostratified ciliated columnar epithelium and renal proximal tubule S3 segment cells. Within the ECD, human CD203c shares 81% aa sequence identity with mouse and rat CD203.
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