Human ER beta /NR3A2 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
IC7106G
Detection of ER beta /NR3A2 in MCF‑7 Human Cell Line by Flow Cytometry.
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Human ER beta /NR3A2 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human ER beta /NR3A2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human ER alpha is observed.
Source
Monoclonal Mouse IgG1 Clone # 733930
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human ER beta /NR3A2
Met1-Gly318
Accession # Q92731
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of ER beta /NR3A2 antibody in MCF‑7 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of ER beta /NR3A2 in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human ER beta /NR3A2 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC7106G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ER beta/NR3A2

Estrogen Receptor beta (ER beta ; also known as NR3A2) is a member of the steroid receptor family. The natural ligand for ER is the classical estrogenic compound 17 beta ‑estradiol. ER beta is expressed in the granulosa cell layer of primary, secondary and mature follicles in the ovary, in bone, bladder, uterus, testis, epididymis, gastrointestinal tract, kidney, breast, heart, vessel wall, immune system, lung, pituitary, hippocampus and hypothalamus. Roles for ER beta in the reproductive and cardiovascular systems have been reported, although these are the subject of conflicting reports. ER beta has been postulated to act primarily as a modulator of ER alpha  function. ER beta has been shown to form homodimers as well as heterodimers with ER alpha. Both ER alpha and ER beta can give rise to numerous isoforms.

Long Name
Estrogen Receptor beta
Entrez Gene IDs
2100 (Human)
Alternate Names
ER beta; Erb; ER-beta; ESR2; ESR-BETA; ESTRB; estrogen receptor 2 (ER beta); estrogen receptor beta 4; estrogen receptor beta; NR3A2; NR3A2ESRB; Nuclear receptor subfamily 3 group A member 2

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Citation for Human ER beta /NR3A2 Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Protumor role of estrogen receptor expression in oral squamous cell carcinoma cells
    Authors: R Akyu Takei, K Tomihara, M Yamazaki, R Moniruzzam, W Heshiki, K Sekido, H Tachinami, K Sakurai, A Yonesi, S Imaue, K Fujiwara, M Noguchi
    Oral surgery, oral medicine, oral pathology and oral radiology, 2021-04-30;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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