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Human ERK3 Alexa Fluor® 750-conjugated Antibody

Catalog #: AF3196S Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3196S-100UG

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ERK3 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects endogenous human ERK3 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human ERK3
Met344-Asn721
Accession # Q16659
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: ERK3

Extracellular signal-regulated kinase 3 (ERK3) is a serine/threonine protein kinase also known as mitogen-activated protein kinase 6 (MAPK6) and p97MAPK. Unlike ERK1 and ERK2, ERK3 lacks the phosphoacceptor sequence T-E-Y in its activation loop. This kinase also possesses a unique C-terminal extension that is missing in traditional MAPKs. Highly unstable, ERK3 is rapidly degraded following N-terminal ubiquitination.

Long Name
Extracellular Signal-regulated Kinase 3
Entrez Gene IDs
5597 (Human); 50772 (Mouse); 58840 (Rat)
Alternate Names
DKFZp686F03189; EC 2.7.11; EC 2.7.11.24; ERK3; ERK-3; ERK3p97-MAPK; Extracellular signal-regulated kinase 3; extracellular signal-regulated kinase, p97; HsT17250; MAP kinase 6; MAP kinase isoform p97; MAPK 6; MAPK6; mitogen-activated protein kinase 6; p97MAPK; PRKM6; protein kinase, mitogen-activated 5; protein kinase, mitogen-activated 6

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