Human ESAM PE-conjugated Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human ESAM in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse ESAM is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 408519
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human ESAM
    Gln30-Ala247
    Accession # Q96AP7
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of ESAM in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human ESAM PE‑conjugated Monoclonal Antibody (Catalog # FAB4204P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ESAM

Endothelial cell Selective Adhesion Molecule (ESAM) is a 55 kDa type I transmembrane glycoprotein that belongs to the JAM family of immunoglobulin superfamily molecules (1, 2). Human ESAM is synthesized as a 390 amino acid (aa) protein composed of a 29 aa signal peptide, a 216 aa extracellular region, a putative 26 aa transmembrane segment, and a 119 aa cytoplasmic domain. The extracellular region contains one V-type and one C2-type Ig domain and is involved in homophilic adhesion (1). In the cytoplasmic domain, there is a docking site for the multifunctional adaptor protein MAGI-1 (3). The extracellular region of human ESAM shows 90%, 74%, 69%, and 67% aa identity with monkey, canine, mouse, and rat extracellular ESAM, respectively. ESAM is expressed on endothelial cells, activated platelets, and megakaryocytes and can be found associated with cell-to-cell junctions. Whether ESAM is restricted to a particular junctional type is not clear (1, 2). ESAM deficient mice have no defect in vascularization but do have reduced angiogenic potential. This may be due to a decreased migratory response to FGF-2 (4).

  • References:
    1. Hirata, K-I. et al. (2001) J. Biol. Chem. 276:16223.
    2. Nasdala, I. et al. (2002) J. Biol. Chem. 277:16294.
    3. Wegmann, F. et al. (2004) Exp. Cell Res. 300:121.
    4. Ishida, T. et. al. (2003) J. Biol. Chem. 278:34598.
  • Long Name:
    Endothelial Cell Adhesion Molecule
  • Entrez Gene IDs:
    90952 (Human); 69524 (Mouse)
  • Alternate Names:
    2310008D05Rik; endothelial cell adhesion molecule; endothelial cell-selective adhesion molecule; ESAM; HUEL (C4orf1)-interacting protein; LP4791 protein; W117m
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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B PE-conjugated Antibody

Ctrl IC0041P 24  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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