Human F-box protein 15/FBXO15 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
IC6035G-100UG
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Human F-box protein 15/FBXO15 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human F‑box protein 15/FBXO15 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse F-box protein 15 is observed.
Source
Monoclonal Mouse IgG2a Clone # 612834
Immunogen
E. coli-derived recombinant human F‑box protein 15/FBXO15 isoform 2

Asn298-Tyr434
Accession # Q8NCQ5-2
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
BG01V human embryonic stem cells fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: F-box protein 15/FBXO15

FBXO15 (F-box only protein 15; also FBX15) is a 50‑60 kDa member of the F-box family, O-subfamily of molecules that is most closely related to FBXO8 and O11. It is found in pluripotent stem cells and testis tissue, and apparently serves as a substrate recognition component of the SCF-type E3 ubiquitin ligase. Human FBXO15 (isoform 2 of accession Q8NCQ5) is 434 amino acids (aa) in length. It contains one F-box (aa 1‑41) that likely serves as an interaction domain for SKP1. There are two potential isoforms, one that contains an alternative start site 76 aa upstream of the standard site (isoform 1 of accession Q8NCQ5), and a second that shows complex splicing that includes a 66 aa insertion after Asn35, a Ser substitution for aa 186‑256, and a 106 aa substitution for aa 304‑346. Over aa 298‑434, human FBXO15 shares 53% aa identity with mouse FBXO15.

Entrez Gene IDs
201456 (Human); 361354 (Rat)
Alternate Names
F-box only protein 15; Fbox protein 15; F-box protein 15; FBX15; FBX15MGC39671; FBXO15

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