Human F-box protein 15/FBXO15 Antibody

Catalog # Availability Size / Price Qty
MAB6035
MAB6035-SP
Human F-box protein 15/FBXO15 Antibody in Data
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Human F-box protein 15/FBXO15 Antibody Summary

Species Reactivity
Human
Specificity
Detects human F‑box protein 15/FBXO15 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse F-box protein 15 is observed.
Source
Monoclonal Mouse IgG2A Clone # 612834
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human F‑box protein 15/FBXO15 isoform 2

Asn298-Tyr434
Accession # Q8NCQ5-2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry F‑box protein 15/FBXO15 in BG01V Human Embryonic Stem Cells. View Larger

F‑box protein 15/FBXO15 in BG01V Human Embryonic Stem Cells. F-box protein 15/FBXO15 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human F-box protein 15/FBXO15 Monoclonal Antibody (Catalog # MAB6035) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007). Oct-3/4 was detected in cell nuclei using Goat Anti-Human Oct-3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759) and stained using NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003). Specific staining of F-box protein 15 was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry Detection of FBXO15 in BG01V Human Stem Cells by Flow Cytometry. View Larger

Detection of FBXO15 in BG01V Human Stem Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Mouse Anti-Human F-box protein 15/FBXO15 Monoclonal Antibody (Catalog # MAB6035, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: F-box protein 15/FBXO15

FBXO15 (F-box only protein 15; also FBX15) is a 50‑60 kDa member of the F-box family, O-subfamily of molecules that is most closely related to FBXO8 and O11. It is found in pluripotent stem cells and testis tissue, and apparently serves as a substrate recognition component of the SCF-type E3 ubiquitin ligase. Human FBXO15 (isoform 2 of accession Q8NCQ5) is 434 amino acids (aa) in length. It contains one F-box (aa 1‑41) that likely serves as an interaction domain for SKP1. There are two potential isoforms, one that contains an alternative start site 76 aa upstream of the standard site (isoform 1 of accession Q8NCQ5), and a second that shows complex splicing that includes a 66 aa insertion after Asn35, a Ser substitution for aa 186‑256, and a 106 aa substitution for aa 304‑346. Over aa 298‑434, human FBXO15 shares 53% aa identity with mouse FBXO15.

Entrez Gene IDs
201456 (Human); 361354 (Rat)
Alternate Names
F-box only protein 15; Fbox protein 15; F-box protein 15; FBX15; FBX15MGC39671; FBXO15

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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