Human Fc gamma  RI/CD64 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB12571P

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Detection of Fc gamma  RI/CD64 in Human Monocytes by Flow Cytometry.
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Human Fc gamma  RI/CD64 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Fc gamma RI in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse (rm) Fc gamma  RI, rmFc gamma  RII, recombinant human (rh) Fc gamma  RIIA, rhFc gamma  RIIB, or rhFc gamma  RIIIB.
Source
Monoclonal Mouse IgG1 Clone # 276426
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Fc gamma  RI
Gln16-Pro288
Accession # P12314.2
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Fc? RI/CD64 antibody in Human Monocytes antibody by Flow Cytometry. View Larger

Detection of Fc gamma RI/CD64 in Human Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human Fc gamma RI/CD64 PE-conjugated Monoclonal Antibody (Catalog # FAB12571P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Fc gamma RI/CD64

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cell-mediated cytotoxicity), cytokine release, and B cell proliferation (1-3). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10‑8 - 10-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10-6 - 10-7 M), only recognizing IgG aggregates surrounding multivalent antigens (1, 4). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains, or associate with one of the ITAM-bearing adapter subunits, Fc R gamma or zeta (3, 5). The only inhibitory member in human and mouse, Fc gamma  RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (5).

Three highly homologous genes (A, B, and C) sharing 98% identity at the nucleotide level have been identified for the human CD64 group (1). Fc gamma RI is transmembrane protein with three extracellular Ig-like domains, and it delivers an activating signal via the associated Fc R gamma accessory chain. The genes for Fc gamma  RIB and Fc gamma  RIC contain stop codons within their membrane proximal Ig-like domains indicating possible secreted receptors (1, 6). An mRNA splice variant of Fc gamma RIB has a deletion of the membrane-proximal Ig-like domain and encodes a putative transmembrane receptor (6). The high affinity recognition of IgG by Fc gamma RI permits the triggering of effector responses at low IgG concentrations typical of early immune responses (2). Fc gamma RI is expressed constitutively on monocytes and macrophages and can be induced on neutrophils and eosinophils (1, 4). Its expression is up-regulated during bacterial infections and sepsis. Over amino acids 16-288, human CD64 shares 72% amino acid sequence identity with mouse CD64.

References
  1. Van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215. 
  2. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181.
  3. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  4. Takai, T. (2002) Nat. Rev. Immunol. 2:580.
  5. Ravetch, J. and L. Lanier (2000) Science 290:84.
  6. Ernst, L. et al. (1998) Mol. Immunol. 35:943.
Long Name
Fc gamma Receptor I
Entrez Gene IDs
2209 (Human); 14129 (Mouse); 295279 (Rat); 102147198 (Cynomolgus Monkey)
Alternate Names
CD64 antigen; CD64; CD64a; Fc fragment of IgG, high affinity Ia, receptor (CD64); Fc gamma RI; FCG1; Fc-gamma receptor I B2; Fc-gamma RI; Fc-gamma RIA; FcgammaRIa; FCGR1; FcgRI; FcgRIA; FCRI; FcRIA; FLJ18345; high affinity Ia, receptor for (CD64); high affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I

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Citations for Human Fc gamma  RI/CD64 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Chemically synthesized molecules with the targeting and effector functions of antibodies.
    Authors: McEnaney P, Fitzgerald K, Zhang A, Douglass E, Shan W, Balog A, Kolesnikova M, Spiegel D
    J Am Chem Soc, 2014;136(52):18034-43.
    Species: N/A
    Sample Types: Protein
    Applications: Binding Assay
  2. Immunophenotypic characterization of human monocyte subsets: possible implications for cardiovascular disease pathophysiology.
    Authors: Shantsila E, Wrigley B, Tapp L, Apostolakis S, Montoro-Garcia S, Drayson MT, Lip GY
    J. Thromb. Haemost., 2011;9(5):1056-66.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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