Human FGF‑BP Alexa Fluor™ Plus 405‑conjugated Antibody

R&D Systems | Catalog # AF1593AFP405

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor Plus 405 (Excitation = 404 nm, Emission = 455 nm)

Antibody Source

Polyclonal Goat IgG
View all FGF-BP Primary Antibodies »

Product Specifications

Specificity

Detects human FGF-BP in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant rat FGF-BP is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human FGF‑BP Alexa Fluor™ Plus 405‑conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: FGF-BP

Fibroblast growth factor binding protein (FGF‑BP), also known as HBp17, is a secreted glycoprotein that increases the bioavailability of FGFs (1). Mature FGF-BP is a 34 kDa, 211 amino acid (aa) O-glycosylated protein with five conserved intrachain disulfide bonds (2‑4). FGF-BP contains a heparin-binding domain (aa 110‑143) and a distinct FGF-binding region (aa 193‑243) (5). Mature human FGF‑BP shares 59% and 54% aa sequence identity with mouse and rat FGF-BP, respectively. FGF-BP is expressed throughout development and in adult squamous epithelium (2, 6). It is upregulated in injured skin, renal tubular epithelium, and spinal nerves as well as in carcinomas of the skin, colon, and pancreas (3, 7‑10). FGF-BP binds FGF-1, -2, -7, -10, and -22 which are secreted and sequestered in the extracellular matrix (ECM) (7, 11). The association of FGF-BP with heparan sulfate proteoglycans (HSPG) weakens HSPG attachment of FGFs and promotes their release (2, 8, 12, 13). FGF‑BP enhances the mitogenic effects of FGFs, thereby contributing to epithelial, endothelial, and neuronal tissue repair, angiogenesis, and tumor growth (7‑9, 11, 14, 15).

References

  1. Abuharbeid, S. et al. (2006) Int. J. Biochem. Cell Biol. 38:1463.
  2. Wu, D. et al. (1991) J. Biol. Chem. 266:16778.
  3. Tassi, E. et al. (2006) Cancer Res. 66:1191.
  4. Lametsch, R. et al. (2000) J. Biol. Chem. 275:19469.
  5. Xie, B. et al. (2006) J. Biol. Chem. 281:1137.
  6. Aigner, A. et al. (2002) Histochem. Cell Biol. 117:1.
  7. Beer, H-D. et al. (2005) Oncogene 24:5269.
  8. Ray, P.E. et al. (2006) Am. J. Physiol. Regul. Integr. Comp. Physiol. 290:R105.
  9. Tassi, E. et al. (2007) Am. J. Physiol. Regul. Integr. Comp. Physiol. 293:R775.
  10. Kurtz, A. et al. (2004) Neoplasia 6:595.
  11. Tassi, E. et al. (2001) J. Biol. Chem. 276:40247.
  12. Mongiat, M. et al. (2001) J. Biol. Chem. 276:10263.
  13. Kurtz, A. et al. (1997) Oncogene 14:2671.
  14. Aigner, A. et al. (2001) Int. J. Cancer 92:510.
  15. Czubayko, F. et al. (1997) Nat. Med. 3:1137.

Long Name

Fibroblast Growth Factor Binding Protein

Alternate Names

FGF-BP1, FGFBP, FGFBP-1, FGFBP1, HBp17

Entrez Gene IDs

9982 (Human); 14181 (Mouse); 64535 (Rat)

Gene Symbol

FGFBP1

UniProt

Q14512

Additional FGF-BP Products

Product Documents for Human FGF‑BP Alexa Fluor™ Plus 405‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human FGF‑BP Alexa Fluor™ Plus 405‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Related Research Areas

Developmental Biology
FGF Family

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