Human Fibromodulin/FMOD PE-conjugated Antibody

IC5945P has been discontinued. View all Fibromodulin/FMOD products.
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Fibromodulin/FMOD in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Lumican is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 549302
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Fibromodulin/FMOD
    Asp75-Ile376
    Accession # NP_002014
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of Fibromodulin/FMOD in
MG-63 Human Cell Line by Flow Cytometry.
MG‑63 human osteosarcoma cell line was stained with Mouse Anti-Human Fibromodulin/FMOD PE‑conjugated Monoclonal Antibody (Catalog # IC5945P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Fibromodulin/FMOD
Fibromodulin (FMOD) is a Class II small leucine-rich proteoglycan (SLRP) superfamily member. The ~50‑70 kDa protein contains a sulfated N‑terminal complement C1q binding domain (aa 19‑98) and 12 leucine-rich repeats (aa 75‑376). A 40 kDa form is reported in dental tissues. It participates in collagen fibrillogenesis in many tissues and competes with Lumican for collagen binding. It can participate in inflammation by activating complement. In epidermal keratinocytes, it may be located in the cytoplasm. The region of human FMOD used as an immunogen shares 96% aa sequence identity with mouse and rat FMOD.
  • Entrez Gene IDs:
    2331 (Human); 14264 (Mouse); 64507 (Rat)
  • Alternate Names:
    Collagen-binding 59 kDa protein; fibromodulin proteoglycan; Fibromodulin; FM; FMOD; Keratan sulfate proteoglycan fibromodulin; SLRR2E; SLRR2EKSPG fibromodulin
Related Research Areas

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