Detection of Fibromodulin/FMOD in MG-63 Human Cell Line by Flow Cytometry.
MG‑63 human osteosarcoma cell line was stained with Mouse Anti-Human Fibromodulin/FMOD PE‑conjugated Monoclonal Antibody (Catalog # IC5945P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Fibromodulin (FMOD) is a Class II small leucine-rich proteoglycan (SLRP) superfamily member. The ~50‑70 kDa protein contains a sulfated N‑terminal complement C1q binding domain (aa 19‑98) and 12 leucine-rich repeats (aa 75‑376). A 40 kDa form is reported in dental tissues. It participates in collagen fibrillogenesis in many tissues and competes with Lumican for collagen binding. It can participate in inflammation by activating complement. In epidermal keratinocytes, it may be located in the cytoplasm. The region of human FMOD used as an immunogen shares 96% aa sequence identity with mouse and rat FMOD.
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