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Human Fibronectin 2nd Generation Cartridge

Name: Human Fibronectin 2nd Generation Cartridge
Brand: R&D Systems
SKU: SPCKB-PS-015771
Price: 450 USD
Availability: InStock

R&D Systems | Catalog # SPCKB-PS-015771

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Product Documents

Simple Plex Human Fibronectin assay kit for use on the Ella instrument. Contains Cartridge, Sample Diluent SD06, and Wash Buffer.

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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

Customize This Cartridge

Compatible Instruments
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (10 µL), Urine (10 µL), Cerebrospinal Fluid (10 µL)

Sensitivity

0.0038 ng/mL

Assay Range

0.01-121 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Cerebrospinal Fluid)

Human Fibronectin 2nd Generation Cartridge Summary

Simple Plex assay is for the detection of human Fibronectin in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), cerebrospinal fluid (CSF), saliva, and urine in Sample Diluent SD06.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Fibronectin Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human Fibronectin and Fibronectin fragments aa 1-1908 and aa 607-1265.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Fibronectin assay.

Inter-Assay Precision (Precision between assays) Replicates of each Fibronectin control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Fibronectin.

Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Saliva, Serum, Urine

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	18	21
Mean (ng/mL)	1.00	45.2	2.38	106
Standard Deviation	0.024	1.51	0.230	7.74
CV%	2.4	3.3	9.7	7.3

Recovery for Human Fibronectin 2nd Generation Cartridge

Recovery for the human Fibronectin assays was evaluated at three different spiked concentrations of Fibronectin protein.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	109	106-113
Urine (n=4)	117	111-120

Linearity

Samples containing and/or spiked with high concentrations of Fibronectin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Images for Human Fibronectin 2nd Generation Cartridge

Simple Plex Human Fibronectin Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Fibronectin Assay. Assay range is 0.01-121 ng/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Fibronectin

Fibronectin (FN) is a large modular glycoprotein that is found as a polymeric fibrillar network in the extracellular matrix (ECM) and as soluble disulfide-linked dimeric protomers in plasma and other body fluids. Fibronectin is a ligand for many molecules including fibrin, heparin, chondroitin sulfate, collagen/gelatin, and integrins. It is involved in multiple cellular processes such as cell adhesion/migration, blood clotting, morphogenesis, tissue repair, and cell signaling. Fibronectin functions are mediated by the insoluble polymeric fibrils. Conversion of soluble fibronectin to fibronectin fibrils in the ECM is initiated by binding to cell surface integrins, resulting in exposure of cryptic epitopes necessary for polymerization.