Human FLI1 Alexa Fluor® 750-conjugated Antibody

Catalog #: AF6474S Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6474S-100UG

Save 15% on Select RUO Reagents. See Details.

All FLI1 Antibodies

Product Details

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FLI1 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human FLI1 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human FLI1
Ser13-Pro112
Accession # Q01543

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: FLI1

FLI1 (Friend leukemia virus integration site 1; also ERGB) is a member of the ETS family of transcription factors. Although its predicted MW is 51 kDa. FLI1 is expressed by megakaryocytes, macrophages, B cells and embryonic endothelial cells. It is a transcriptional activator that regulates the genes for Tie-2, GpIIb, MPL and TGF-beta RII. Human FLI1 is 452 amino acids (aa) in length. It contains one PNT domain that is involved in heterotypic interactions (aa 112-198) and an ETS DNA‑binding domain (aa 281-361). There are two potential splice variants. One shows an alternative start site at Met34, while another contains a 10 aa substitution for aa 1‑76. There are a series of naturally occurring 62-68 kDa chimeric proteins (EWS/FLI1) in tumors that result from a chromosomal translocation involving the EWS and FLI1 genes. One creates a 499 aa chimera that includes aa 1-265 of EWS and 220-452 of FLI1. Over aa 13-112, human FLI1 shares 98% aa identity with mouse FLI1.

Long Name

Friend Leukemia Integration 1

Entrez Gene IDs

2313 (Human); 14247 (Mouse); 315532 (Rat)

Alternate Names

ERGB; EWSR2; FLI1; Friend leukemia integration 1 transcription factor; Friend leukemia virus integration 1; Proto-oncogene Fli-1; SIC-1; Transcription factor ERGB