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Human FoxC2 Alexa Fluor® 594-conjugated Antibody

Catalog #: FAB5044T Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB5044T-100UG

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Human FoxC2 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human FoxC2 in direct ELISAs and Western blots. Approximately 25% cross-reactivity with recombinant human (rh) FoxA1 (aa 253‑472) is observed in Western blots but not in direct ELISAs. In direct ELISAs and Western blots, no cross-reactivity w
Source
Monoclonal Mouse IgG2a Clone # 665819
Purification
Protein A or G purified
Immunogen
E. coli-derived recombinant human FoxC2
Gly415-Tyr501
Accession # Q99958
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 
Immunocytochemistry
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: FoxC2

FoxC2 belongs to a large family of nuclear transcription factor proteins sharing a common forkhead/winged helix DNA binding domain. FoxC2 is implicated in epithelial to mesenchymal transition, human lymphedemia-distichiasis syndrome, and tumor metastasis. Experiments in mice indicate that FoxC2 controls adipocyte morphogenesis and null mice show defects in axial and cranial skeletogenesis, as well. In addition the transcriptional activity of FoxC2 influences expression of cytokine receptors such as CXCR4.

Long Name
Forkhead Box C2
Entrez Gene IDs
2303 (Human); 14234 (Mouse)
Alternate Names
Fkh14; FKHL14LD; forkhead box C2 (MFH-1, mesenchyme forkhead 1); forkhead, Drosophila, homolog-like 14; Forkhead-related protein FKHL14; FoxC2; LD; Mesenchyme fork head protein 1; mesenchyme forkhead 1; MFH-1 protein; MFH1; MFH-1; MFH1forkhead box protein C2; Transcription factor FKH-14

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