Human FoxC2 Antibody (665819)

R&D Systems | Catalog # MAB5044

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 665819
View all FoxC2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FoxC2
Gly415-Tyr501
Accession # Q99958

Specificity

Detects human FoxC2 in direct ELISAs and Western blots. Approximately 25% cross-reactivity with recombinant human (rh) FoxA1 (aa 253‑472) is observed in Western blots but not in direct ELISAs. In direct ELISAs and Western blots, no cross-reactivity with rhFoxB1 (aa 191‑324), D3 (aa 1-140), F1 (aa 129-354), J1 (aa 306-421), K1 (aa 493-670), M1 (aa 642-763), P1 (aa 231-420), P2 (aa 269-460), P3 (aa 179‑279), or P4 (aa 229-410) is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human FoxC2 Antibody (665819)

Detection of Human FoxC2 antibody by Western Blot.

Detection of Human FoxC2 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, T47D human breast cancer cell line, and MDA-MB-453 human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Human FoxC2 Monoclonal Antibody (Catalog # MAB5044) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FoxC2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
FoxC2 antibody in MDA-MB-453 Human Cell Line by Immunocytochemistry (ICC).

FoxC2 in MDA‑MB‑453 Human Cell Line.

FoxC2 was detected in immersion fixed MDA-MB-453 human breast cancer cell line using Human FoxC2 Monoclonal Antibody (Catalog # MAB5044) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human FoxC2 Antibody (665819)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MDA‑MB‑453 human breast cancer cell line

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, T47D human breast cancer cell line, and MDA‑MB‑453 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FoxC2

FoxC2 belongs to a large family of nuclear transcription factor proteins sharing a common forkhead/winged helix DNA binding domain. FoxC2 is implicated in epithelial to mesenchymal transition, human lymphedemia-distichiasis syndrome, and tumor metastasis. Experiments in mice indicate that FoxC2 controls adipocyte morphogenesis and null mice show defects in axial and cranial skeletogenesis, as well. In addition the transcriptional activity of FoxC2 influences expression of cytokine receptors such as CXCR4.

Long Name

Forkhead Box C2

Alternate Names

Fkh14, LD, MFH1

Entrez Gene IDs

2303 (Human); 14234 (Mouse)

Gene Symbol

FOXC2

UniProt

Q99958

Additional FoxC2 Products

Product Documents for Human FoxC2 Antibody (665819)

Certificate of Analysis

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Product Specific Notices for Human FoxC2 Antibody (665819)

For research use only

Citations for Human FoxC2 Antibody (665819)

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Protocols

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