Human FoxC2 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human FoxC2 in direct ELISAs and Western blots. Approximately 25% cross-reactivity with recombinant human (rh) FoxA1 (aa 253‑472) is observed in Western blots but not in direct ELISAs. In direct ELISAs and Western blots, no cross-reactivity with rhFoxB1 (aa 191‑324), D3 (aa 1-140), F1 (aa 129-354), J1 (aa 306-421), K1 (aa 493-670), M1 (aa 642-763), P1 (aa 231-420), P2 (aa 269-460), P3 (aa 179‑279), or P4 (aa 229-410) is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 665819
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human FoxC2
    Gly415-Tyr501
    Accession # Q99958
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human FoxC2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, T47D human breast cancer cell line, and MDA‑MB‑453 human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Human FoxC2 Monoclonal Antibody (Catalog # MAB5044) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FoxC2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
FoxC2 in MDA‑MB‑453 Human Cell Line. FoxC2 was detected in immersion fixed MDA‑MB‑453 human breast cancer cell line using Human FoxC2 Monoclonal Antibody (Catalog # MAB5044) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FoxC2
FoxC2 belongs to a large family of nuclear transcription factor proteins sharing a common forkhead/winged helix DNA binding domain. FoxC2 is implicated in epithelial to mesenchymal transition, human lymphedemia-distichiasis syndrome, and tumor metastasis. Experiments in mice indicate that FoxC2 controls adipocyte morphogenesis and null mice show defects in axial and cranial skeletogenesis, as well. In addition the transcriptional activity of FoxC2 influences expression of cytokine receptors such as CXCR4.
  • Long Name:
    Forkhead Box C2
  • Entrez Gene IDs:
    2303 (Human); 14234 (Mouse)
  • Alternate Names:
    Fkh14; FKHL14LD; forkhead box C2 (MFH-1, mesenchyme forkhead 1); forkhead, Drosophila, homolog-like 14; Forkhead-related protein FKHL14; FoxC2; LD; Mesenchyme fork head protein 1; mesenchyme forkhead 1; MFH-1 protein; MFH1; MFH-1; MFH1forkhead box protein C2; Transcription factor FKH-14
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