Human Fucosyltransferase 3/5 (FUT3/5) Lumenal Domain Alexa Fluor® 594-conjugated Antibody

Catalog #: AF4068T Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4068T-100UG

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Product Details

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Human Fucosyltransferase 3/5 (FUT3/5) Lumenal Domain Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human FUT3 and human FUT5 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human FUT5
Arg35-Thr374
Accession # Q11128

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Fucosyltransferase 3/FUT3

FUT5 ( alpha -1, 3-fucosyltransferase 5; also Fuc-TV) is a 43 kDa member of the glycosyltransferase 10 family. It is responsible for the terminal step in the synthesis of Le^x, sialyl‑Le^x, and Le^a antigens. This is accomplished by the transfer of fucose to preexisting LacNAc or sialyl-LacNAc structures. Human FUT5 is 374 amino acids (aa) in length. It is a type II transmembrane Golgi glycoprotein with a C-terminal lumenal domain (aa’s 35-374). The catalytic domain spans aa’s 77-374, while the acceptor-binding motif includes aa’s 120-126. FUT5 is the product of a single exon gene. Thus, there are no splice variants. FUT5 only occurs in primates; there is no rodent ortholog.

Entrez Gene IDs

2525 (Human)

Alternate Names

CD174; FT3B; Fucosyltransferase 3; FucT-III; FUT3; LE; Les; Lewis FT