Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Sheep IgG
View all Fucosyltransferase 8/FUT8 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Fucosyltransferase 8/FUT8
Asp32-Lys575
Accession # Q9BYC5

Specificity

Detects human Fucosyltransferase 8/FUT8 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) FUT3, rhFUT5, and rhFUT11 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human Fucosyltransferase 8/FUT8 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Fucosyltransferase 8/FUT8 (Catalog # 5768-GT)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fucosyltransferase 8/FUT8

FUT8 (Fucosyltransferase 8; also alpha 1,6 Fucosyltransferase) is a 60‑65 kDa member of the glycosyltransferase 23 family of enzymes. It catalyzes the addition of fucose to Asn-linked GlcNAc via an alpha 1,6 linkage. FUT8 is widely expressed, and the effects of its fucosylation vary. A lack of fucosylation on IgG potentiates ADCC activity, while an absence of fucose on the EGF R and alpha 3 beta 1 integrin decreases their activity. Human FUT8 is a type II transmembrane protein 575 amino acids (aa) in length. It contains a short cytoplasmic region (aa 1‑9) plus an extended luminal domain (aa 31‑575) that possesses one SH3 homology domain (aa 502‑563). There are at least four potential splice variants. There is an alternate start site at Met479, a 14 aa substitution for aa 1‑420, a 32 aa substitution for aa 1‑161, and a two aa substitution for aa 444‑575. Over aa 32‑575, human FUT8 shares 97% aa identity with mouse FUT8.

Alternate Names

alpha 1,6 Fucosyltransferase, alpha1-6FucT, FUT8

Entrez Gene IDs

2530 (Human); 53618 (Mouse); 432392 (Rat)

Gene Symbol

FUT8

UniProt

Q9BYC5

Additional Fucosyltransferase 8/FUT8 Products

Product Documents for Human Fucosyltransferase 8/FUT8 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Fucosyltransferase 8/FUT8 Biotinylated Antibody

For research use only

Related Research Areas

Glycobiology-related Enzymes

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