Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western, Immunoprecipitation

Cited:

Immunohistochemistry, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Fucosyltransferase 8/FUT8 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Fucosyltransferase 8/FUT8 
Asp32-Lys575
Accession # Q9BYC5

Specificity

Detects human Fucosyltransferase 8/FUT8 in direct ELISAs.  In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) FUT3, rhFUT5, and rhFUT11 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Fucosyltransferase 8/FUT8 Antibody

Fucosyltransferase 8/FUT8 antibody in Human Colon by Immunohistochemistry (IHC-P).

Fucosyltransferase 8/FUT8 in Human Colon.

Fucosyltransferase 8/FUT8 was detected in paraffin-embedded sections of human colon using Sheep Anti-Human Fucosyltransferase 8/FUT8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5768) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Fucosyltransferase 8/FUT8 antibody by Western Blot.

Detection of Human Fucosyltransferase 8/FUT8 by Western Blot.

Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Fucosyltransferase 8/FUT8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5768) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Fucosyltransferase 8/FUT8 at approximately 60 - 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Human Fucosyltransferase 8/FUT8 antibody by Simple WesternTM.

Detection of Human Fucosyltransferase 8/FUT8 by Simple WesternTM.

Simple Western lane view shows lysates of COLO 205 human colorectal adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Fucosyltransferase 8/FUT8 at approximately 64 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Fucosyltransferase 8/FUT8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5768) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Fucosyltransferase 8/FUT8 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Fucosyltransferase 8/FUT8, see our available Western blot detection antibodies

Simple Western

10 µg/mL
Sample: COLO 205 human colorectal adenocarcinoma cell line

Western Blot

1 µg/mL
Sample: COLO 205 human colorectal adenocarcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fucosyltransferase 8/FUT8

FUT8 (Fucosyltransferase 8; also alpha 1,6 Fucosyltransferase) is a 60‑65 kDa member of the glycosyltransferase 23 family of enzymes. It catalyzes the addition of fucose to Asn-linked GlcNAc via an alpha 1,6 linkage. FUT8 is widely expressed, and the effects of its fucosylation vary. A lack of fucosylation on IgG potentiates ADCC activity, while an absence of fucose on the EGF R and alpha 3 beta 1 integrin decreases their activity. Human FUT8 is a type II transmembrane protein 575 amino acids (aa) in length. It contains a short cytoplasmic region (aa 1‑9) plus an extended luminal domain (aa 31‑575) that possesses one SH3 homology domain (aa 502‑563). There are at least four potential splice variants. There is an alternate start site at Met479, a 14 aa substitution for aa 1‑420, a 32 aa substitution for aa 1‑161, and a two aa substitution for aa 444‑575. Over aa 32‑575, human FUT8 shares 97% aa identity with mouse FUT8.

Alternate Names

alpha 1,6 Fucosyltransferase, alpha1-6FucT, FUT8

Entrez Gene IDs

2530 (Human); 53618 (Mouse); 432392 (Rat)

Gene Symbol

FUT8

UniProt

Q9BYC5

Additional Fucosyltransferase 8/FUT8 Products

Product Documents for Human Fucosyltransferase 8/FUT8 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Fucosyltransferase 8/FUT8 Antibody

For research use only

Related Research Areas

Glycobiology-related Enzymes

Citations for Human Fucosyltransferase 8/FUT8 Antibody

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Protocols

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