Human Fucosyltransferase 8/FUT8 Antibody

Catalog # Availability Size / Price Qty
AF5768
AF5768-SP
Fucosyltransferase 8/FUT8 in Human Colon.
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Citations (3)
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Human Fucosyltransferase 8/FUT8 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Fucosyltransferase 8/FUT8 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Fucosyltransferase 8/FUT8 
Asp32-Lys575
Accession # Q9BYC5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Fucosyltransferase 8/FUT8, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Fucosyltransferase 8/FUT8 antibody in Human Colon by Immunohistochemistry (IHC-P). View Larger

Fucosyltransferase 8/FUT8 in Human Colon. Fucosyltransferase 8/FUT8 was detected in paraffin-embedded sections of human colon using Sheep Anti-Human Fucosyltransferase 8/FUT8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5768) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot Detection of Human Fucosyltransferase 8/FUT8 antibody by Western Blot. View Larger

Detection of Human Fucosyltransferase 8/FUT8 by Western Blot. Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Fucosyltransferase 8/FUT8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5768) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Fucosyltransferase 8/FUT8 at approximately 60 - 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Simple Western Detection of Human Fucosyltransferase 8/FUT8 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human Fucosyltransferase 8/FUT8 by Simple WesternTM. Simple Western lane view shows lysates of COLO 205 human colorectal adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Fucosyltransferase 8/FUT8 at approximately 64 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Fucosyltransferase 8/FUT8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5768) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fucosyltransferase 8/FUT8

FUT8 (Fucosyltransferase 8; also alpha 1,6 Fucosyltransferase) is a 60‑65 kDa member of the glycosyltransferase 23 family of enzymes. It catalyzes the addition of fucose to Asn-linked GlcNAc via an alpha 1,6 linkage. FUT8 is widely expressed, and the effects of its fucosylation vary. A lack of fucosylation on IgG potentiates ADCC activity, while an absence of fucose on the EGF R and alpha 3 beta 1 integrin decreases their activity. Human FUT8 is a type II transmembrane protein 575 amino acids (aa) in length. It contains a short cytoplasmic region (aa 1‑9) plus an extended luminal domain (aa 31‑575) that possesses one SH3 homology domain (aa 502‑563). There are at least four potential splice variants. There is an alternate start site at Met479, a 14 aa substitution for aa 1‑420, a 32 aa substitution for aa 1‑161, and a two aa substitution for aa 444‑575. Over aa 32‑575, human FUT8 shares 97% aa identity with mouse FUT8.

Entrez Gene IDs
2530 (Human); 53618 (Mouse); 432392 (Rat)
Alternate Names
alpha 1,6 Fucosyltransferase; alpha-(1,6)-fucosyltransferase; alpha1-6FucT; EC 2.4.1.68; fucosyltransferase 8 (alpha (1,6) fucosyltransferase); Fucosyltransferase 8; FUT8; GDP-fucose--glycoprotein fucosyltransferase; GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,6-fucosyltransferase; Glycoprotein 6-alpha-L-fucosyltransferase; MGC26465

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Citations for Human Fucosyltransferase 8/FUT8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. The BNT162b2 mRNA SARS-CoV-2 vaccine induces transient afucosylated IgG1 in naive but not in antigen-experienced vaccinees
    Authors: J Van Coilli, T Pongracz, J Rahmöller, HJ Chen, CE Geyer, LA van Vught, JS Buhre, T Šušti?, TLJ van Osch, M Steenhuis, W Hoepel, W Wang, AS Lixenfeld, J Nouta, S Keijzer, F Linty, R Visser, MD Larsen, EL Martin, I Künsting, S Lehrian, V von Kopylo, C Kern, HB Lunding, M de Winther, N van Mourik, T Rispens, T Graf, MA Slim, RP Minnaar, MK Bomers, JJ Sikkens, APJ Vlaar, CE van der Sc, J den Dunnen, M Wuhrer, M Ehlers, G Vidarsson, Fatebenefr, UMC COVID-
    EBioMedicine, 2022-12-16;87(0):104408.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. FUT8-mediated aberrant N-glycosylation of B7H3 suppresses the immune response in triple-negative breast cancer
    Authors: Y Huang, HL Zhang, ZL Li, T Du, YH Chen, Y Wang, HH Ni, KM Zhang, J Mai, BX Hu, JH Huang, LH Zhou, D Yang, XD Peng, GK Feng, J Tang, XF Zhu, R Deng
    Nature Communications, 2021-05-11;12(1):2672.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. A Comprehensive Analysis of FUT8 Overexpressing Prostate Cancer Cells Reveals the Role of EGFR in Castration Resistance
    Authors: N Höti, TS Lih, J Pan, Y Zhou, G Yang, A Deng, L Chen, M Dong, RB Yang, CF Tu, MC Haffner, Q Kay Li, H Zhang
    Cancers (Basel), 2020-02-18;12(2):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

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