Detects human Galectin-3 in direct ELISAs.
In direct ELISAs, 100% cross-reactivity
with recombinant mouse Galectin-3 is observed and no cross-reactivity with
recombinant human (rh) Galectin-8 or rhGalectin-9 is observed.
Monoclonal Mouse IgG2B Clone # 194801
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Galectin-3 Ala2-Ile250 Accession # P17931
Supplied in a saline solution containing BSA and Sodium Azide.
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Galectin‑3 in Human Blood Monocytes by Flow Cytometry. LPS-treated peripheral blood monocytes were stained with Mouse Anti-Human Galectin‑3 PE‑conjugated Monoclonal Antibody (Catalog # IC11542P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Galectin-3, also known as Mac-2, L29, CBP35 and IgE Binding Protein, is a member of a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their Carbohydrate Recognition Domains (CRD), have been identified to date. Galectin-3 is expressed in tumor cells, macrophages, activated T cells, osteoclasts, epithelial cells and fibroblasts where it forms homodimers, or heterodimers, likely with DMBT1. It is reported to complex with Integrins alpha3 and beta1. Galectin-3 is known to circulate and show elevation in inflammatory disorders.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.