Detects human Galectin-3 in direct ELISAs.
In direct ELISAs, 100% cross-reactivity
with recombinant mouse Galectin-3 is observed and no cross-reactivity with
recombinant human (rh) Galectin-8 or rhGalectin-9 is observed.
Monoclonal Mouse IgG2B Clone # 194801
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Galectin-3 Ala2-Ile250 Accession # P17931
Supplied in a saline solution containing BSA and Sodium Azide.
Detection of Galectin‑3 in Human Blood Monocytes by Flow Cytometry.
LPS-treated peripheral blood monocytes were stained with Mouse Anti-Human Galectin‑3 PE‑conjugated Monoclonal Antibody (Catalog # IC11542P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Galectin-3, also known as Mac-2, L29, CBP35 and IgE Binding Protein, is a member of a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their Carbohydrate Recognition Domains (CRD), have been identified to date. Galectin-3 is expressed in tumor cells, macrophages, activated T cells, osteoclasts, epithelial cells and fibroblasts where it forms homodimers, or heterodimers, likely with DMBT1. It is reported to complex with Integrins alpha3 and beta1. Galectin-3 is known to circulate and show elevation in inflammatory disorders.
Have you used Human Galectin-3 PE-conjugated Antibody?
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