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Human GDF-15 Quantikine QuicKit ELISA

Catalog #: QK957 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
QK957
Control Products Available
QC282: Human GDF-15 Quantikine QuicKit Control Group 282
Human GDF-15 Standard Curve
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human GDF-15 Quantikine QuicKit ELISA Summary

Assay Length
80 minutes
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Urine (10 uL)
Sensitivity
0.947 pg/mL
Assay Range
23.4 - 1,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Recognizes natural and recombinant human GDF-15
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

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Human GDF-15 Quantikine QuicKit Control Group 282
Cat # QC282

Product Summary

The Quantikine™ QuicKit™ Human GDF-15 Immunoassay is a one step, 80-minute solid phase ELISA designed to measure human GDF-15 levels in cell culture supernates, serum, plasma, and urine. It contains CHO cell-expressed recombinant human GDF-15 and antibodies raised against the recombinant protein. Results obtained using natural human GDF-15 showed linear curves that were parallel to the standard curves obtained using the QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural human GDF-15.

Precision

Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least three technicians

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 20 20 10 10
Mean (pg/mL) 156 882 165 925
Standard Deviation 6.12 58.8 9.49 77.6
CV% 3.9 6.7 5.7 8.4

Recovery

The recovery of human GDF-15 spiked to three levels in samples throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 92-113
EDTA Plasma (n=2) 74 66-83
Heparin Plasma (n=2) 77 66-93
Serum (n=2) 77 66-84
Urine (n=2) 104 94-116

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human GDF-15 were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human GDF-15 Linearity

Scientific Data

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Human GDF-15 Standard Curve

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Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: GDF-15

Growth/differentiation factors (GDF-1 to GDF-15) are members of the BMP family of TGF-beta superfamily proteins. They are produced as inactive preproproteins which are then cleaved and assembled into active secreted homodimers. GDF dimers are disulfide-linked with the exception of GDF-3 and -9. GDF proteins are important during embryonic development, particularly in the skeletal, nervous, and muscular systems.

Long Name:
Growth Differentiation Factor 15
Entrez Gene IDs:
9518 (Human); 23886 (Mouse); 29455 (Rat)
Alternate Names:
GDF15; GDF-15; growth differentiation factor 15; growth/differentiation factor 15; Macrophage inhibitory cytokine 1; MIC-1; MIC-1NSAID-activated gene 1 protein; MIC1Prostate differentiation factor; NAG-1; NAG-1NSAID-regulated gene 1 protein; NSAID (nonsteroidal inflammatory drug)-activated protein 1; PDF; PDFGDF-15; PLAB; PLABNRG-1; Placental bone morphogenetic protein; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta

FAQs

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ELISA Controls

Human GDF-15 Quantikine QuicKit Control Group 282

QC282
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