Human GPR15 PE-conjugated Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Stains human GPR15-transfected cells but not irrelevant transfectants.
  • Source
    Monoclonal Mouse IgG2B Clone # 367902
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human GPR15
    Met1-Leu360
    Accession # P49685
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of GPR15 in HEK293 Human Cell Line Transfected with Human GPR15 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human GPR15 and eGFP was stained with either (A) Mouse Anti-Human GPR15 PE‑conjugated Monoclonal Antibody (Catalog # FAB3654P) or (B) Mouse IgG2B Phycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: GPR15
GPR15, also known as BOB, is a seven-transmembrane G protein-coupled receptor that is expressed in CD4+ T cells and alveolar macrophages. GPR15 functions as a cellular co-receptor for some isolates of HIV-1, HIV-2, and SIV through interactions with several viral envelope proteins. Human GPR15 shares 96%‑100% amino acid sequence identity with chimpanzee, macaque, and rhesus GPR15, and 76% with mouse GPR15.
  • Long Name:
    G Protein-coupled Receptor 15
  • Entrez Gene IDs:
    2838 (Human); 71223 (Mouse)
  • Alternate Names:
    Bob; Brother of Bonzo; G protein-coupled receptor 15; GPR15; G-protein coupled receptor 15; MGC126828; MGC126830
Related Research Areas

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B PE-conjugated Antibody

Ctrl IC0041P 24  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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