Granulysin, formerly known as NKG5 or Lymphokine LAG-2, is a membrane-lytic protein that is expressed in granules of natural killer and activated cytotoxic T cells. It exhibits cytolytic activity against intracellular or extracellular microbes and also tumors, either alone or in synergy with Perforin. IL-15 is necessary and sufficient for Granulysin release from CD8+ T cells. Granulysin promotes chemotaxis and increases production of chemokines by monocytic cells as well as target cell lysis. It binds and disrupts microbial membranes through charge interactions.
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Human Granulysin Quantikine QuicKit ELISA
R&D Systems | Catalog # QK3138
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Key Product Details
Assay Length
80 minutes
Sample Type & Volume Required Per Well
Cell Culture Supernates (13 µL), Cell Lysates (13 µL), Serum (13 µL), EDTA Plasma (13 µL), Heparin Plasma (13 µL)
Sensitivity
1.43 pg/mL
Assay Range
18.8-1200 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma)
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Product Summary for Human Granulysin Quantikine QuicKit ELISA
The Quantikine QuicKit Human Granulysin Immunoassay is a one-step, 80-minute solid phase ELISA designed to measure human Granulysin levels in cell culture supernates, serum, plasma, and lysates.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Human
Specificity
Natural and recombinant human Granulysin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least six technicians.
Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 20 | 20 | 10 | 10 |
| Mean (pg/mL) | 110 | 636 | 87.1 | 571 |
| Standard Deviation | 8.26 | 18.3 | 8.0 | 37.8 |
| CV% | 7.5 | 2.9 | 9.2 | 6.6 |
Recovery for Human Granulysin Quantikine QuicKit ELISA
The recovery of Granulysin is spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 110 | 97-131 |
| EDTA Plasma (n=2) | 96 | 84-106 |
| Heparin Plasma (n=2) | 112 | 99-121 |
| Lysis Buffer (n=1) | 106 | 96-114 |
| Serum (n=2) | 108 | 92-122 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Granulysin were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Granulysin Quantikine QuicKit ELISA
Quantikine QuicKit Human Granulysin Immunoassay Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Granulysin
Additional Granulysin Products
Product Documents for Human Granulysin Quantikine QuicKit ELISA
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Granulysin Quantikine QuicKit ELISA
For research use only
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