Human HTRA1/PRSS11 Antibody

Catalog #: AF2916 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2916
AF2916-SP

Detection of Human HTRA1/PRSS11 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HTRA1/PRSS11 Antibody Summary

Species Reactivity

Human

Specificity

Detects human HTRA1/PRSS11 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human HTRA1/PRSS11
Gly156-Pro480
Accession # Q92743

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human HTRA1/PRSS11 by Western Blot. Western blot shows lysates of JAR human choriocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human HTRA1/PRSS11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2916) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for HTRA1/PRSS11 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human HTRA1/PRSS11 by Simple Western^TM. Simple Western lane view shows lysates of JAR human choriocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for HTRA1/PRSS11 at approximately 54 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human HTRA1/PRSS11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2916) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HTRA1/PRSS11

HTRA1 is a member of the mammalian HTRA (high temperature requirement A) serine protease family. The E. coli HTRA homolog functions as a chaperone-protease and is essential for bacterial survival at temperatures above 42 °C (1). Among the four mammalian HTRA proteins, HTRA1, -3 and -4 are secreted while HTRA2 is localized in mitochondria. HTRA1 contains an N-terminal insulin-like growth factor binding protein (IGFBP) domain, a Kazal-type trypsin inhibitor motif, and C-terminal trypsin-like protease and PDZ domains (2). It is involved in several pathologies including Alzheimer’s disease (3), rheumatoid arthritis (4), osteoarthritis (5) and age‑related macular degeneration (6), although the mechanisms by which HTRA1 exerts its effects are not clear. HTRA1 also has properties of a tumor suppressor protein, which makes it a target for cancer therapy (7). In addition HTRA1 is known to regulate the TGF-beta signaling pathway and bone mineralization (8, 9). rhHTRA1 lacks the N-terminal IGFBP and Kazal-like domains, but retains the serine protease and PDZ domains.

References

Jiang, J. et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105:11939.
Murwantoko et al. (2004) Biochem. J. 381:895.
Grau, S. et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102:6021.
Grau, S. et al. (2006) J. Biol. Chem. 281:6124.
Hu, S. et al. (1998) J. Biol. Chem. 273:34406.
Dewan, A. et al. (2006) Science 314:989.
Chien, J. et al. (2004) Oncogene 23:1636.
Oka, C. et al. (2004) Development 131:1041.
Hadfield, K.D. et al. (2008) J. Biol. Chem. 283:5928.

Long Name

High Temperature Requirement Protein-1

Entrez Gene IDs

5654 (Human)

Alternate Names

ARMD7; EC 3.4.21; EC 3.4.21.108; High-temperature requirement A serine peptidase 1; HtrA serine peptidase 1; HTRA1; IGFBP5-protease; L56; ORF480; PRSS11; Serine protease 11; serine, 11 (IGF binding)