Human HTRA1/PRSS11 Antibody

R&D Systems | Catalog # AF2916

R&D Systems
Discontinued Product
AF2916 has been discontinued. View all HTRA1/PRSS11 products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Simple Western

Cited:

ELISA Capture

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all HTRA1/PRSS11 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human HTRA1/PRSS11
Gly156-Pro480
Accession # Q92743

Specificity

Detects human HTRA1/PRSS11 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human HTRA1/PRSS11 Antibody

Detection of Human HTRA1/PRSS11 antibody by Western Blot.

Detection of Human HTRA1/PRSS11 by Western Blot.

Western blot shows lysates of JAR human choriocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human HTRA1/PRSS11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2916) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for HTRA1/PRSS11 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human HTRA1/PRSS11 antibody by Simple WesternTM.

Detection of Human HTRA1/PRSS11 by Simple WesternTM.

Simple Western lane view shows lysates of JAR human choriocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for HTRA1/PRSS11 at approximately 54 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human HTRA1/PRSS11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2916) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human HTRA1/PRSS11 Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: JAR human choriocarcinoma cell line

Western Blot

1 µg/mL
Sample: JAR human choriocarcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HTRA1/PRSS11

HTRA1 is a member of the mammalian HTRA (high temperature requirement A) serine protease family. The E. coli HTRA homolog functions as a chaperone-protease and is essential for bacterial survival at temperatures above 42 °C (1). Among the four mammalian HTRA proteins, HTRA1, -3 and -4 are secreted while HTRA2 is localized in mitochondria. HTRA1 contains an N-terminal insulin-like growth factor binding protein (IGFBP) domain, a Kazal-type trypsin inhibitor motif, and C-terminal trypsin-like protease and PDZ domains (2). It is involved in several pathologies including Alzheimer’s disease (3), rheumatoid arthritis (4), osteoarthritis (5) and age‑related macular degeneration (6), although the mechanisms by which HTRA1 exerts its effects are not clear. HTRA1 also has properties of a tumor suppressor protein, which makes it a target for cancer therapy (7). In addition HTRA1 is known to regulate the TGF-beta signaling pathway and bone mineralization (8, 9). rhHTRA1 lacks the N-terminal IGFBP and Kazal-like domains, but retains the serine protease and PDZ domains.

References

  1. Jiang, J. et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105:11939.
  2. Murwantoko et al. (2004) Biochem. J. 381:895.
  3. Grau, S. et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102:6021.
  4. Grau, S. et al. (2006) J. Biol. Chem. 281:6124.
  5. Hu, S. et al. (1998) J. Biol. Chem. 273:34406.
  6. Dewan, A. et al. (2006) Science 314:989.
  7. Chien, J. et al. (2004) Oncogene 23:1636.
  8. Oka, C. et al. (2004) Development 131:1041.
  9. Hadfield, K.D. et al. (2008) J. Biol. Chem. 283:5928.

Long Name

High Temperature Requirement Protein-1

Alternate Names

IGFBP5-protease, PRSS11

Entrez Gene IDs

5654 (Human)

Gene Symbol

HTRA1

UniProt

Q92743

Additional HTRA1/PRSS11 Products

Product Documents for Human HTRA1/PRSS11 Antibody

Certificate of Analysis

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Product Specific Notices for Human HTRA1/PRSS11 Antibody

For research use only

Related Research Areas

Serine Proteases and Regulators

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