Human HTRA1/PRSS11 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human HTRA1/PRSS11 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human HTRA2 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human HTRA1/PRSS11
    Gly156-Pro480
    Accession # Q92743
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human HTRA1/PRSS11 by Western Blot. Western blot shows lysates of JAR human choriocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human HTRA1/PRSS11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2916) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for HTRA1/PRSS11 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human HTRA1/PRSS11 by Simple WesternTM. Simple Western lane view shows lysates of JAR human choriocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for HTRA1/PRSS11 at approximately 54 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human HTRA1/PRSS11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2916) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HTRA1/PRSS11
HTRA1 is a member of the mammalian HTRA (high temperature requirement A) serine protease family. The E. coli HTRA homolog functions as a chaperone-protease and is essential for bacterial survival at temperatures above 42 °C (1). Among the four mammalian HTRA proteins, HTRA1, -3 and -4 are secreted while HTRA2 is localized in mitochondria. HTRA1 contains an N-terminal insulin-like growth factor binding protein (IGFBP) domain, a Kazal-type trypsin inhibitor motif, and C-terminal trypsin-like protease and PDZ domains (2). It is involved in several pathologies including Alzheimer’s disease (3), rheumatoid arthritis (4), osteoarthritis (5) and age‑related macular degeneration (6), although the mechanisms by which HTRA1 exerts its effects are not clear. HTRA1 also has properties of a tumor suppressor protein, which makes it a target for cancer therapy (7). In addition HTRA1 is known to regulate the TGF-beta signaling pathway and bone mineralization (8, 9). rhHTRA1 lacks the N-terminal IGFBP and Kazal-like domains, but retains the serine protease and PDZ domains.
  • References:
    1. Jiang, J. et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105:11939.
    2. Murwantoko et al. (2004) Biochem. J. 381:895.
    3. Grau, S. et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102:6021.
    4. Grau, S. et al. (2006) J. Biol. Chem. 281:6124.
    5. Hu, S. et al. (1998) J. Biol. Chem. 273:34406.
    6. Dewan, A. et al. (2006) Science 314:989.
    7. Chien, J. et al. (2004) Oncogene 23:1636.
    8. Oka, C. et al. (2004) Development 131:1041.
    9. Hadfield, K.D. et al. (2008) J. Biol. Chem. 283:5928.
  • Long Name:
    High Temperature Requirement Protein-1
  • Entrez Gene IDs:
    5654 (Human)
  • Alternate Names:
    ARMD7; EC 3.4.21; EC 3.4.21.108; High-temperature requirement A serine peptidase 1; HtrA serine peptidase 1; HTRA1; IGFBP5-protease; L56; ORF480; PRSS11; Serine protease 11; serine, 11 (IGF binding)
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