Human IBSP/Sialoprotein II Antibody Summary
Phe17-Gln317
Accession # AAC95490
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
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Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IBSP/Sialoprotein II
IBSP (integrin-binding sialoprotein; also BSP or bone sialoprotein (II)) is a 55‑75 kDa, secreted, variably glycosylated, monomeric noncollagenous member of the SIBLING family of extracellular matrix (ECM) proteins (1‑3). It is principally associated with the early stages of bone mineralization. BSP is synthesized as a 317 amino acid (aa) precursor that contains a 16 aa signal sequence and a 301 aa mature region (4‑6). The mature segment is divided into a basic N-terminus (aa 17‑62), a central region (aa 63‑233), and an acidic C‑terminus (aa 234‑317) (7).
Functional segments associated with the mature molecule include a type I collagen binding domain (aa 19‑46), two non‑RGD cell binding sites (aa 30‑57 and 261‑281), an RGD alpha v beta 3 integrin‑binding site (aa 286‑288) and two potential hydroxyapatite (HAp) nucleation domains (aa 76‑83 and 151‑158) (3, 4, 8‑11). HAp formation requires a BSP nucleation site composed of at least eight consecutive glutamic acid residues and, likely, a contribution from a BSP‑associated co‑nucleator (10, 12). BSP is highly glycosylated, sulfated, and phosphorylated. Phosphorylation may impact HAp growth, while carbohydrate may regulate cell adhesion (1, 3, 13). Mature human BSP is 70%, 72%, 78%, and 72% aa identical to porcine, rat, canine, and mouse BSP, respectively. BSP is synthesized by megakaryocytes/platelets, osteoblasts, osteocytes, odontoblasts, osteoclasts, and bone marrow stromal cells (14‑17).
- Qin, C. et al. (2004) Crit. Rev. Oral Biol. Med. 15:126.
- Alford, A.I. and K.D. Hankenson (2006) Bone 38:749.
- Ganss, B. et al. (1999) Crit. Rev. Oral Biol. Med. 10:79.
- Fisher, L.W. et al. (1990) J. Biol. Chem. 265:2347.
- Kerr, J.M. et al. (1993) Genomics 17:408.
- Kim, R.H. et al. (1994) Matrix Biol. 14:31.
- Zaia, J. et al. (2001) Biochemistry 40:12983.
- Tye, C.E. et al. (2005) J. Biol. Chem. 280:13487.
- Stubbs, J.T. et al. (1997) J. Bone Miner. Res. 12:1210.
- Tye, C.E. et al. (2003) J. Biol. Chem. 278:7949.
- Miyauchi, A. et al. (1991) J. Biol. Chem. 266:20369.
- Hakki, S.S. et al. (2006) J. Periodontol. 77:167.
- Wazen, R.M. et al. (2007) J. Histochem. Cytochem. 55:35.
- Kacena, M.A. et al. (2006) Bone 39:978.
- Bianco, P. et al. (1991) Calcif. Tissue Int. 49:421.
- Chen, J. et al. (1992) J. Bone Miner. Res. 7:987.
- Kreke, M.R. et al. (2005) Bone 36:1047.
Product Datasheets
Citations for Human IBSP/Sialoprotein II Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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VCAM-1 Targeted Lipopolyplexes as Vehicles for Efficient Delivery of shRNA-Runx2 to Osteoblast-Differentiated Valvular Interstitial Cells; Implications in Calcific Valve Disease Treatment
Authors: G Voicu, D Rebleanu, CA Mocanu, G Tanko, I Droc, CM Uritu, M Pinteala, I Manduteanu, M Simionescu, M Calin
International Journal of Molecular Sciences, 2022-03-30;23(7):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Exosomal miR-19a and IBSP cooperate to induce osteolytic bone metastasis of estrogen receptor-positive breast cancer
Authors: K Wu, J Feng, F Lyu, F Xing, S Sharma, Y Liu, SY Wu, D Zhao, A Tyagi, RP Deshpande, X Pei, MG Ruiz, H Takahashi, S Tsuzuki, T Kimura, YY Mo, Y Shiozawa, R Singh, K Watabe
Nature Communications, 2021-08-31;12(1):5196.
Species: Human
Sample Types:
Applications: Binding Assay -
Nano-Polyplexes Mediated Transfection of Runx2-shRNA Mitigates the Osteodifferentiation of Human Valvular Interstitial Cells
Authors: G Voicu, D Rebleanu, CA Constantin, EV Fuior, L Ciortan, I Droc, CM Uritu, M Pinteala, I Manduteanu, M Simionescu, M Calin
Pharmaceutics, 2020-06-02;12(6):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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