Human IL‑18/IL‑1F4 Antibody

Name: Human IL‑18/IL‑1F4 Antibody
Brand: R&D Systems
SKU: D045-3
Price: 489 USD
Availability: InStock

R&D Systems | Catalog # D045-3

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Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunoprecipitation

Cited:

Luminex Development

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG_2A Clone # 159-12B

View all IL-18/IL-1F4 Primary Antibodies »

Product Specifications

Immunogen

Human IL-18/IL-1F4

Specificity

This antibody reacts with human IL-18 on Immunoprecipitation.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Applications for Human IL‑18/IL‑1F4 Antibody

Application

Recommended Usage

Immunoprecipitation

5 μg/ 0.5 μg recombinant human IL-18

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

100 μg IgG in 100 μL PBS containing 50% glycerol, pH 7.2. Contains no preservatives.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

This antibody solution is stable for one year from the date of purchase when stored at -20°C.

Background: IL-18/IL-1F4

Interleukin 18 (IL-18) is a 18 kDa cytokine which identified as a costimulatory factor for production of interferon-gamma (IFN-gamma ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-gamma is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-gamma activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens, and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-gamma. Pleiotropic effects of IL-18 has also been reported, such as, enhancement production of IFN-gamma and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-gamma in T cells, enhancement of Fas ligand expression by Th1 cells.