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Human IL-2 Fluorescein-conjugated Antibody

Catalog #: IC202F
3 Citations Datasheet / COA / SDS
Newer Version Available: IC202G
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Discontinued Product

IC202F has been discontinued and is replaced by IC202T. IC202S. IC202G. IC202R. IC202U. IC202N.

Detection of IL‑2 in Human PBMCs treated with PMA and Ca2+ionomycin by Flow Cytometry.
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Citations (3)
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Human IL-2 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-2 in direct ELISAs. Does not cross-react with recombinant IL-2 from mouse, rat, pig, or cotton rat.
Source
Monoclonal Mouse IgG1 Clone # 5334
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IL‑2
Ala21-Thr153
Accession # NP_000577
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of IL-2 antibody in Human PBMCs treated with PMA and Ca<sup>2+</sup>ionomycin antibody by Flow Cytometry. View Larger

Detection of IL‑2 in Human PBMCs treated with PMA and Ca2+ionomycin by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were treated with PMA and Ca2+ionomycin stained with Mouse Anti-Human IL-2 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC202F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IL-2

IL-2 is a 15-19 kDa secreted O-glycosylated polypeptide that belongs to the gamma c -receptor utilizing family of molecules. It is a monomer that is expressed by a limited number of cell types, including CD4+ and CD8+ T cells, gamma δ T cells, eosinophils and B cells. Its local concentration, plus the stoichiometry of it receptor ( a two or three subunit complex) appears to determine what effects it has on its target cells. It is suggested to both induce NK and CD8+ T cell proliferation, and promote NK cell and CD8+ T cell effector activity. IL-2 also appears to drive CD4+ Fox P3- thymocytes into mature FoxP3+ Tregs, and to direct the conversion of CD4+ T cells into induced Tregs. Finally, IL-2 induces gamma δ T cells to secrete IFN-gamma, and endothelial cells to upregulate endocytic activity. Human and mouse IL-2 share 56 % amino acid sequence identity.

Long Name
Interleukin 2
Entrez Gene IDs
3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
Alternate Names
Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; Proleukin; T cell growth factor; T-cell growth factor; TCGF

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Citations for Human IL-2 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Expansion of anti-mesothelin specific CD4+ and CD8+ T cell responses in patients with pancreatic carcinoma.
    Authors: Chen Y, Ayaru L, Mathew S, Morris E, Pereira S, Behboudi S
    PLoS ONE, 2014-02-10;9(2):e88133.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Expression of the T cell receptor alpha/beta on a CD123+ BDCA2+ HLA-DR+ subpopulation in head and neck squamous cell carcinoma.
    Authors: Thiel A, Kesselring R, Pries R, Puzik A, Wittkopf N, Wollenberg B
    PLoS ONE, 2011-01-11;6(1):e15997.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Analysis of CD4+ T-Cell responses to a novel alpha-fetoprotein-derived epitope in hepatocellular carcinoma patients.
    Authors: Alisa A, Ives A, Pathan AA, Navarrete CV, Williams R, Bertoletti A, Behboudi S
    Clin. Cancer Res., 2005-09-15;11(18):6686-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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