Human IL-2 Fluorescein-conjugated Antibody

(3 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-2 in direct ELISAs. Does not cross-react with recombinant IL-2 from mouse, rat, pig, or cotton rat.
  • Source
    Monoclonal Mouse IgG1 Clone # 5334
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human IL‑2
    Ala21-Thr153
    Accession # NP_000577
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of IL‑2 in Human PBMCs treated with PMA and Ca2+ ionomycin by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were treated with PMA and Ca2+ ionomycin stained with Mouse Anti-Human IL‑2 Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC202F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-2

IL-2 is a 15-19 kDa secreted O-glycosylated polypeptide that belongs to the gamma c -receptor utilizing family of molecules. It is a monomer that is expressed by a limited number of cell types, including CD4+ and CD8+ T cells, gamma δ T cells, eosinophils and B cells. Its local concentration, plus the stoichiometry of it receptor ( a two or three subunit complex) appears to determine what effects it has on its target cells. It is suggested to both induce NK and CD8+ T cell proliferation, and promote NK cell and CD8+ T cell effector activity. IL-2 also appears to drive CD4+ Fox P3- thymocytes into mature FoxP3+ Tregs, and to direct the conversion of CD4+ T cells into induced Tregs. Finally, IL-2 induces gamma δ T cells to secrete IFN-gamma, and endothelial cells to upregulate endocytic activity. Human and mouse IL-2 share 56 % amino acid sequence identity.

  • Long Name:
    Interleukin 2
  • Entrez Gene IDs:
    3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
  • Alternate Names:
    aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Expansion of anti-mesothelin specific CD4+ and CD8+ T cell responses in patients with pancreatic carcinoma.
    Authors: Chen Y, Ayaru L, Mathew S, Morris E, Pereira S, Behboudi S
    PLoS ONE, 2014;9(2):e88133.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Expression of the T cell receptor alpha/beta on a CD123+ BDCA2+ HLA-DR+ subpopulation in head and neck squamous cell carcinoma.
    Authors: Thiel A, Kesselring R, Pries R, Puzik A, Wittkopf N, Wollenberg B
    PLoS ONE, 2011;6(1):e15997.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Analysis of CD4+ T-Cell responses to a novel alpha-fetoprotein-derived epitope in hepatocellular carcinoma patients.
    Authors: Alisa A, Ives A, Pathan AA, Navarrete CV, Williams R, Bertoletti A, Behboudi S
    Clin. Cancer Res., 2005;11(18):6686-94.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Fluorescein-conjugated Antibody

Ctrl IC002F 14  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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