Human IL-31 RA Alexa Fluor® 405-conjugated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-31 RA in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse IL-31 RA is observed.
  • Source
    Monoclonal Rat IgG1 Clone # 313308
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0-derived rhIL-31 RA extracellular domain
    aa 20-516
    Accession # NP_620586
  • Formulation
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 405
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25-1 µg/106 cells
    U937 human histiocytic lymphoma cell line (untreated) or human peripheral blood mononuclear cells (PBMCs) treated with Recombinant Human IFN‑ gamma (Catalog # 285-IF)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-31 RA

The interleukin-31 receptor A subunit (IL-31 RA), also known as gp130-Like Monocyte Receptor (GLM-R or GPL), is a ~100 kDa type I transmembrane glycoprotein that is classified as being a type I cytokine receptor (1, 2). A heterodimeric complex of IL-31 RA and the oncostatin M receptor (OSM-R) functions as the signaling receptor for IL-31 (3). Both subunits are inducibly expressed throughout the myelomonocytic lineage and are upregulated by interferon-gamma and bacterial lipopolysaccharides (1-3). IL-31 RA is also expressed on keratinocytes, dorsal root ganglia neurons, and variably on lung epithelial cells (3-6). The 732 amino acid (aa) IL-31 RA contains a 19 aa signal sequence, a 500 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 192 aa cytoplasmic domain. The ECD shares 60%, 58%, 73% and 70% aa identity with mouse, rat, canine and bovine IL-31 RA ECD, respectively. Human IL-31 receptors do not respond to mouse IL-31 (7). The ECD contains five fibronectin type III domains; the first two contain four conserved cysteine residues and a WSXWS motif common to type I cytokine receptors (2). Twelve alternately spliced human IL-31 RA isoforms are known and range in size from 356-745 amino acids. A long (745 aa) and a short (560 aa) transmembrane form are the predominant forms, and many cell lines express both forms (8). The long form, like the 732 aa form, signals by recruiting STAT3, 5 or 1, while the short form does not recruit STATs and inhibits IL-31 signaling. The ratio of these forms and their co‑expression with OSM-R determines a cell's response to IL‑31 (8). In both humans and transgenic mice, IL-31 from skin-homing Th2 cells may contribute to the pruritis (itching) associated with nonatopic dermatitis, especially in infected skin (3, 9, 10).

  • References:
    1. Ghilardi, N. et al. (2002) J. Biol. Chem. 277:16831.
    2. Diveu, C. et al. (2003) J. Biol. Chem. 278:49850.
    3. Dillon, S. R. et al. (2004) Nat. Immunol. 5:752.
    4. Chattopadhyay, S. et al. (2007) J. Biol. Chem. 282:3014.
    5. Perrigoue, J. G. et al. (2007) J. Exp. Med. 204:481.
    6. Bando, T. et al. (2006) Neuroscience 142:1263.
    7. Broxmeyer, H. E. et al. (2007) Exp. Hematol. 35:78.
    8. Diveu, C. et al. (2004) Eur. Cytokine. Netw. 15:291.
    9. Bilsborough, J. et al. (2006) J. Allergy Clin. Immunol. 117:418.
    10. Sonkoly, E. et al. (2006) J. Allergy Clin. Immunol. 117:411.
  • Long Name:
    Interleukin 31 Receptor A
  • Entrez Gene IDs:
    133396 (Human); 218624 (Mouse)
  • Alternate Names:
    class I cytokine receptor; CRL3; CRL3Glmr; Cytokine receptor-like 3; GLMR; GLM-R; GLM-RMGC125346; GP130 like receptor; Gp130-like monocyte receptor; Gp130-like receptor; hGLM-R; IL-31 RA; IL-31 receptor subunit alpha; IL-31R subunit alpha; IL31RA; IL-31RA; IL-31RAGPLCRL; IL-31R-alpha; interleukin 31 receptor A; interleukin-31 receptor subunit alpha; PRO21384; soluble type I cytokine receptor CRL3; zcytoR17
Related Research Areas
Isotype Controls
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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