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Rat IgG1 Alexa Fluor® 405-conjugated Antibody

Catalog #: IC005V Datasheet / COA / SDS

Discontinued Product

IC005V has been discontinued.
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Rat IgG1 Alexa Fluor® 405-conjugated Antibody Summary

Specificity
Selected for use as rat IgG1 isotype control for flow cytometry applications.
Source
Monoclonal Rat IgG1 Clone # 43414
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 5 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months, 2 to 8 °C under sterile conditions after opening.

    • Background: IgG1

    R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

    Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

    Long Name
    Immunoglobulin G1
    Alternate Names
    IgG1; Igh-4; RGD1359539; VH7183

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    FAQs

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