Human IL-31RA Alexa Fluor® 405-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB2769V-100UG
R&D Systems Antibodies
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human IL-31RA Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-31 RA in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse IL-31 RA is observed.
Source
Monoclonal Rat IgG1 Clone # 313308
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0-derived rhIL-31 RA extracellular domain
aa 20-516
Accession # NP_620586
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
U937 human histiocytic lymphoma cell line (untreated) or human peripheral blood mononuclear cells (PBMCs) treated with Recombinant Human IFN‑ gamma (Catalog # 285-IF)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-31RA

The interleukin-31 receptor A subunit (IL-31 RA), also known as gp130-Like Monocyte Receptor (GLM-R or GPL), is a ~100 kDa type I transmembrane glycoprotein that is classified as being a type I cytokine receptor (1, 2). A heterodimeric complex of IL-31 RA and the oncostatin M receptor (OSM-R) functions as the signaling receptor for IL-31 (3). Both subunits are inducibly expressed throughout the myelomonocytic lineage and are upregulated by interferon-gamma and bacterial lipopolysaccharides (1-3). IL-31 RA is also expressed on keratinocytes, dorsal root ganglia neurons, and variably on lung epithelial cells (3-6). The 732 amino acid (aa) IL-31 RA contains a 19 aa signal sequence, a 500 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 192 aa cytoplasmic domain. The ECD shares 60%, 58%, 73% and 70% aa identity with mouse, rat, canine and bovine IL-31 RA ECD, respectively. Human IL-31 receptors do not respond to mouse IL-31 (7). The ECD contains five fibronectin type III domains; the first two contain four conserved cysteine residues and a WSXWS motif common to type I cytokine receptors (2). Twelve alternately spliced human IL-31 RA isoforms are known and range in size from 356-745 amino acids. A long (745 aa) and a short (560 aa) transmembrane form are the predominant forms, and many cell lines express both forms (8). The long form, like the 732 aa form, signals by recruiting STAT3, 5 or 1, while the short form does not recruit STATs and inhibits IL-31 signaling. The ratio of these forms and their co‑expression with OSM-R determines a cell's response to IL‑31 (8). In both humans and transgenic mice, IL-31 from skin-homing Th2 cells may contribute to the pruritis (itching) associated with nonatopic dermatitis, especially in infected skin (3, 9, 10).

References
  1. Ghilardi, N. et al. (2002) J. Biol. Chem. 277:16831.
  2. Diveu, C. et al. (2003) J. Biol. Chem. 278:49850.
  3. Dillon, S. R. et al. (2004) Nat. Immunol. 5:752.
  4. Chattopadhyay, S. et al. (2007) J. Biol. Chem. 282:3014.
  5. Perrigoue, J. G. et al. (2007) J. Exp. Med. 204:481.
  6. Bando, T. et al. (2006) Neuroscience 142:1263.
  7. Broxmeyer, H. E. et al. (2007) Exp. Hematol. 35:78.
  8. Diveu, C. et al. (2004) Eur. Cytokine. Netw. 15:291.
  9. Bilsborough, J. et al. (2006) J. Allergy Clin. Immunol. 117:418.
  10. Sonkoly, E. et al. (2006) J. Allergy Clin. Immunol. 117:411.
Long Name
Interleukin 31 Receptor A
Entrez Gene IDs
133396 (Human); 218624 (Mouse)
Alternate Names
class I cytokine receptor; CRL3; CRL3Glmr; Cytokine receptor-like 3; GLMR; GLM-R; GLM-RMGC125346; GP130 like receptor; Gp130-like monocyte receptor; Gp130-like receptor; hGLM-R; IL-31 RA; IL-31 receptor subunit alpha; IL-31R subunit alpha; IL31RA; IL-31RA; IL-31RAGPLCRL; IL-31R-alpha; interleukin 31 receptor A; interleukin-31 receptor subunit alpha; PRO21384; soluble type I cytokine receptor CRL3; zcytoR17

Product Datasheets

You must select a language.

x

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human IL-31RA Alexa Fluor® 405-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human IL-31RA Alexa Fluor® 405-conjugated Antibody and earn rewards!

Have you used Human IL-31RA Alexa Fluor® 405-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review