Human IL-34 PE-conjugated Antibody

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Detection of IL‑34 in NS0 Mouse Cell Line Transfected with Human IL-34 by Flow Cytometry.
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Product Details
Citations (2)

Human IL-34 PE-conjugated Antibody Summary

Species Reactivity
Detects recombinant human IL-34 in direct ELISAs.  In direct ELISAs, no cross-reactivity with recombinant mouse IL-34 is observed.
Monoclonal Mouse IgG1 Clone # 578416
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human IL-34
Accession # Q6ZMJ4
Supplied in a saline solution containing BSA and Sodium Azide.
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)


Recommended Concentration
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of IL‑34 antibody in NS0 Mouse Cell Line Transfected with Human IL-34 antibody by Flow Cytometry. View Larger

Detection of IL‑34 in NS0 Mouse Cell Line Transfected with Human IL-34 by Flow Cytometry. NS0 mouse myeloma cell line transfected with human IL-34 was stained with Mouse Anti-Human IL-34 PE-conjugated Monoclonal Antibody (Catalog # IC5265P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IL-34

Interleukin 34 (IL-34; also known as uncharacterized protein C16orf77) is secreted as a homodimer consisting of 39 kDa monomers (1). It belongs to no known cytokine family. Human IL-34 is synthesized as a 242 amino acid (aa) precursor that contains a 20 aa signal sequence and a 222 aa mature chain. The mature chain contains one potential site of N-linked glycosylation. Human IL-34 is 71% identical to mouse IL-34 on the amino acid level (1). IL-34 is expressed in various tissues, including the heart, brain, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate, and colon, and is most abundant in the spleen (1). The receptor for IL-34 is colony-stimulating factor 1 receptor (CSF-1R) (1,2). IL-34 stimulates monocyte proliferation (1). In functional studies, IL-34, like CSF-1, the other ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1/2) in human monocytes (1). In addition, IL-34 promoted the formation of the colony-forming unit-macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures (1,3).

  1. Lin, H. et al. (2008) Science 320:807.
  2. Chihara, T. et al. (2010) Cell Death Differ. 17:1917.
  3. Wei, S. et al. (2010) J. Leukoc. Biol. 88:495.
Long Name
Interleukin 34
Entrez Gene IDs
146433 (Human); 76527 (Mouse)
Alternate Names
C16orf77; IL34; IL-34; interleukin 34

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Citations for Human IL-34 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Cross-Reactive Donor-Specific CD8+ Tregs Efficiently Prevent Transplant Rejection
    Authors: E Picarda, S Bézie, L Usero, J Ossart, M Besnard, H Halim, K Echasserie, C Usal, J Rossjohn, K Bernardeau, S Gras, C Guillonnea
    Cell Rep, 2019-12-24;29(13):4245-4255.e6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. IL-34 is a Treg-specific cytokine and mediates transplant tolerance.
    Authors: Bezie S, Picarda E, Ossart J, Tesson L, Usal C, Renaudin K, Anegon I, Guillonneau C
    J Clin Invest, 2015-09-21;125(10):3952-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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