Human IL-5 R alpha /CD125 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB253P
Detection of IL‑5 R alpha /CD125 in Human Blood Granulocytes by Flow Cytometry.
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Human IL-5 R alpha /CD125 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-5 R alpha /CD125 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross‑react with recombinant human (rh) IL-5 R beta, rhIL-9 R, rhIL-4 R, recombinant mouse IL-5 R alpha, or rhIL-13 R alpha 1.
Source
Monoclonal Mouse IgG1 Clone # 26815
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑5 R alpha /CD125
Asp21-Arg335
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IL-5 Ra/CD125 antibody in Human Blood Granulocytes antibody by Flow Cytometry. View Larger

Detection of IL‑5 R alpha /CD125 in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human IL-5 Ra/CD125 PE-conjugated Monoclonal Antibody (Catalog # FAB253P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of IL-5 Ra/CD125 antibody in HEK293 Human Cell Line Transfected with Human IL-5 Ra/CD125 and eGFP antibody by Flow Cytometry. View Larger

Detection of IL‑5 R alpha /CD125 in HEK293 Human Cell Line Transfected with Human IL‑5 R alpha /CD125 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human IL-5 Ra/CD125 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human IL-5 Ra/CD125 PE-conjugated Monoclonal Antibody (Catalog # FAB253P). Quadrant markers were set based on control antibody staining (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-5 R alpha/CD125

Interleukin 5, produced primarily by activated T cells and mast cells, has diverse biological effects on a variety of cell types. Human IL-5 is a potent eosinophil differentiation and activation factor in vivo and in vitro. Additionally, it has also been reported that IL-5 can stimulate the proliferation and/or differentiation of basophils and B cells. The multiple effects of IL-5 are mediated by binding of the cytokine to specific cell surface receptors expressed on target cells. As is the case with many other cytokines, the functional high-affinity receptor for IL-5 is a complex consisting of a ligand binding subunit ( alpha chain) and a second subunit ( beta chain) that can modulate the ligand binding affinity of the receptor complex. In the case of IL-5, the beta subunit is shared with the high affinity receptor complexes for IL-3 and GM-CSF. The beta  chain does not bind any of the cytokines in question but is indispensable for the cytokine-mediated signaling. cDNA clones for the alpha  chain (IL-5 R alpha ) of both the mouse and human high affinity IL-5 receptor complexes have been isolated. Human and mouse IL-5 R alpha are both members of the hematopoietin receptor superfamily characterized by the presence of the WSXWS, and a four cysteine residue motif in the extracellular domain of the transmembrane protein. In addition to the cDNA clone encoding the full-length transmembrane protein, cDNA clones that arise from alternative splicing and that encode soluble secreted forms of IL-5 R alpha have been isolated from mouse as well as human cells. A naturally-occurring soluble form of the IL-5 R alpha has been detected in biological fluids of autoimmune-prone mice and mice bearing chronic B cell leukemia (BCL1). A recombinant human IL-5 soluble receptor  alpha has been shown to bind the human IL-5 dimer in a 1:1 ratio and acts as a human ­IL-5 antagonist. This molecule inhibits the proliferation of IL-5-dependent cell lines and blocks human umbilical cord blood eosinophil differentiation.

Long Name
Interleukin 5 Receptor alpha
Entrez Gene IDs
3568 (Human); 16192 (Mouse)
Alternate Names
CD125 antigen; CD125; CDw125CD125; HSIL5R3; IL-5 R alpha; IL-5 receptor subunit alpha; IL5R alpha; IL-5R subunit alpha; IL5R; IL5RA; IL-5Ra; IL-5R-alpha; interleukin 5 receptor type 3; interleukin 5 receptor, alpha; interleukin-5 receptor alpha chain; interleukin-5 receptor alpha subunit; interleukin-5 receptor subunit alpha; MGC26560

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Human IL-5 R alpha /CD125 PE-conjugated Antibody
By Aida Flores on 05/17/2016
Application: Flow Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human