Human Importin  alpha 5/KPNA1 Alexa Fluor™ Plus 647‑conjugated Antibody

R&D Systems | Catalog # AF6206AFP647

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor Plus 647 (Excitation = 658 nm, Emission = 675 nm)

Antibody Source

Polyclonal Sheep IgG
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Product Specifications

Specificity

Detects human Importin alpha 5/KPNA1 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Importin alpha 5/KPNA1

Importin subunit alpha-5 (KPNA1, Karyopherin subunit alpha 1; also SRP1 beta, NPI-1 and RAG2) is a 65 kDa member of the importin alpha family of proteins. It is ubiquitously expressed, and found in both nucleus and cytoplasm. KPNA1 functions as a cargo carrier that transports various complexes from cytoplasm into nucleus. Two scenarios are possible. First, NLS‑containing cargo molecules bind to KPNA1, which then binds to importin beta ; alternatively, KPNA1 and importin beta initially form a complex that primes KPNA1 for subsequent cargo binding. In either case, importin beta binds to the nuclear pore, facilitating transport into the nucleus. Human KPNA1 is 538 amino acids (aa) in length. It contains an N-terminal IBB/importin beta domain (aa 1‑57), ten Armadillo repeats that bind "cargo" (aa 77‑504) and two intervening NLS binding sites. Over aa 1-132, human KPNA1 shares 97% aa identity with mouse KPNA1.

Alternate Names

IPOA5, KPNA1, NPI-1, RCH2, SRP1

Entrez Gene IDs

3836 (Human); 16646 (Mouse); 288064 (Rat)

Gene Symbol

KPNA1

UniProt

Additional Importin alpha 5/KPNA1 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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