Human Importin  alpha 5/KPNA1 Antibody

Catalog # Availability Size / Price Qty
AF6206
AF6206-SP
Detection of Human Importin  alpha 5/KPNA1 by Western Blot.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human Importin  alpha 5/KPNA1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Importin alpha 5/KPNA1 in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Importin alpha 5/KPNA1
Met1-Val132
Accession # P52294
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Importin a5/KPNA1 antibody by Western Blot. View Larger

Detection of Human Importin alpha 5/KPNA1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Importin a5/KPNA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6206) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Importin a5/KPNA1 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Importin alpha 5/KPNA1

Importin subunit alpha-5 (KPNA1, Karyopherin subunit alpha 1; also SRP1 beta, NPI-1 and RAG2) is a 65 kDa member of the importin alpha family of proteins. It is ubiquitously expressed, and found in both nucleus and cytoplasm. KPNA1 functions as a cargo carrier that transports various complexes from cytoplasm into nucleus. Two scenarios are possible. First, NLS‑containing cargo molecules bind to KPNA1, which then binds to importin beta ; alternatively, KPNA1 and importin beta initially form a complex that primes KPNA1 for subsequent cargo binding. In either case, importin beta binds to the nuclear pore, facilitating transport into the nucleus. Human KPNA1 is 538 amino acids (aa) in length. It contains an N-terminal IBB/importin beta domain (aa 1‑57), ten Armadillo repeats that bind "cargo" (aa 77‑504) and two intervening NLS binding sites. Over aa 1-132, human KPNA1 shares 97% aa identity with mouse KPNA1.

Entrez Gene IDs
3836 (Human); 16646 (Mouse); 288064 (Rat)
Alternate Names
Importin alpha 5; importin subunit alpha-1; importin-alpha-S1; IPOA5; karyopherin alpha 1 (importin alpha 5); Karyopherin subunit alpha-1; KPNA1; NPI-1; NPI-1SRP1importin alpha 5; Nucleoprotein interactor 1; RCH2; RCH2RAG cohort protein 2; recombination activating gene cohort 2; SRP1; SRP1-beta

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human Importin  alpha 5/KPNA1 Antibody

There are currently no reviews for this product. Be the first to review Human Importin  alpha 5/KPNA1 Antibody and earn rewards!

Have you used Human Importin  alpha 5/KPNA1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review