Human Indoleamine 2,3-dioxygenase/IDO PerCP-conjugated Antibody

Catalog # Availability Size / Price Qty
IC6030C
Detection of Indoleamine 2,3‑dioxygenase/IDO in Human MDSCs by Flow Cytometry.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human Indoleamine 2,3-dioxygenase/IDO PerCP-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Indoleamine 2,3-dioxygenase/IDO in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 700838
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Indoleamine 2,3-dioxygenase/IDO
Ala2-Gly403
Accession # P14902
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Complete Your Research

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of Indoleamine 2,3-dioxygenase/IDO antibody in Human MDSCs antibody by Flow Cytometry. View Larger

Detection of Indoleamine 2,3‑dioxygenase/IDO in Human MDSCs by Flow Cytometry. Human PBMC-derived myeloid-derived suppressor cells (MDSCs) treated with 10 ng/mL Recombinant Human IL-6 (Catalog # 206-IL) and 10 ng/mL Recombinant Human GM-CSF (Catalog # 215-GM) for 7 days were stained with Mouse Anti-Human Siglec-3/CD33 PE-conjugated Monoclonal Antibody (Catalog # FAB1137P) and either (A) Mouse Anti-Human Indoleamine 2,3-dioxygenase/IDO PerCP-conjugated Monoclonal Antibody (Catalog # IC6030C) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002C). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Indoleamine 2,3-dioxygenase/IDO

Indoleamine 2,3-dioxygenase (IDO) is a heme-containing intracellular dioxygenase catalyzing the degradation of the essential amino acid L-tryptophan to N-formyl-kynurenine (1). This degradation is the first and rate-limiting step of the L-kynurenine pathway (2). IDO is widely expressed in dendritic cells, macrophages, microglia, eosinophils, fibroblasts, endothelial cells, and most tumor cells. In immune cells, its expression is mainly induced by cytokines such as IFN-gamma, IFN-alpha, IFN-beta, and IL‑10. IDO has an antimicrobial function due to its decreasing the availability of the essential amino acid tryptophan in inflammatory environments (3). Recent studies have demonstrated that IDO induces immunosuppression during infection, pregnancy, transplantation, autoimmunity, and neoplasia (3-5).

References
  1. Lewis-Ballester, A. et al. (2009) Proc. Natl. Acad. Sci. USA. 106:17371.
  2. Costantino, G. (2009) Expert Opin. Ther. Targets 13:247.
  3. Xu, H. et al. (2008) Immunol. Lett. 121:1.
  4. Lob, S. et al. (2009) Nat. Rev. Cancer 9:445.
  5. Curti, A. et al. (2009) Blood 113:2394.
Entrez Gene IDs
3620 (Human); 15930 (Mouse)
Alternate Names
3dioxygenase; EC 1.13.11.52; IDO; IDO1; IDOIDO-1; INDO; INDOindole 2,3-dioxygenase; Indoleamine 2; indoleamine 2,3-dioxygenase 1; Indoleamine 2,3-dioxygenase; indoleamine-pyrrole 2,3 dioxygenase; Indoleamine-pyrrole 2,3-dioxygenase

Product Datasheets

You must select a language.

x

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human Indoleamine 2,3-dioxygenase/IDO PerCP-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human Indoleamine 2,3-dioxygenase/IDO PerCP-conjugated Antibody and earn rewards!

Have you used Human Indoleamine 2,3-dioxygenase/IDO PerCP-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review