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Recombinant Human IL-6 Protein

R&D Systems | Catalog # 206-IL

GMP Version Available: 206-GMP
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Key Product Details

  • R&D Systems E. coli-derived Recombinant Human IL-6 Protein (206-IL)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

P05231.1

Applications

Bioactivity
View all IL-6 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human IL-6 protein
Pro29-Met212

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Pro29

Predicted Molecular Mass

20.9 kDa

Activity

Measured in a cell proliferation assay using T1165.85.2.1 mouse plasmacytoma cells. Nordan, R.P. et al. (1987) J. Immunol. 139:813.
The ED50 for this effect is 0.2-0.8 ng/mL.

Reviewed Applications

Read 26 reviews rated 4.7 using 206-IL in the following applications:

Scientific Data Images for Recombinant Human IL-6 Protein

Recombinant Human IL‑6 Protein SEC-MALS.

Recombinant Human IL-6 (Catalog # 206-IL) has a molecular weight (MW) of 23.1 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).
Recombinant Human IL-6 Protein Bioactivity

Recombinant Human IL-6 Protein Bioactivity

Recombinant Human IL-6 (Catalog # 206-IL) stimulates cell proliferation of the T1165.85.2.1 mouse plasmacytoma cell line. The ED50 for this effect is 0.2-0.8 ng/mL.
Recombinant Human IL-6 Protein SDS-PAGE

Recombinant Human IL-6 Protein SDS-PAGE

1 μg/lane of Recombinant Human IL-6 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 21 kDa.

Equivalent Bioactivity of GMP, Animal-Free, and RUO grades of Recombinant Human IL-6

Equivalent bioactivity of GMP (206-GMP), Animal-Free (AFL206), and RUO (Catalog # 206-IL) grades of Recombinant Human IL-6 as measured in cell proliferation assay (orange, green, red, respectively).

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 206-IL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100-200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 206-IL/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution Reconstitute at 100-200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-6

Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, 22-28 kDa phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression (1-5). Mature human IL-6 is 183 amino acids (aa) in length and shares 39% aa sequence identity with mouse and rat IL-6 (6). Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties (7-10). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization (11). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (12). Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage (5). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha (5). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes (2, 5). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor (5, 13). IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease (1-5). When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis (1, 2, 5). IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise (2). In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells (1, 14).

References

  1. Mansell, A. and B.J. Jenkins (2013) Cytokine Growth Factor Rev. 24:249.
  2. Schuett, H. et al. (2009) Thromb. Haemost. 102:215.
  3. Erta, M. et al. (2012) Int. J. Biol. Sci. 8:1254.
  4. Garbers, C. et al. (2012) Cytokine Growth Factor Rev. 23:85.
  5. Mihara, M. et al. (2012) Clin. Sci. (Lond.) 122:143.
  6. Hirano, T. et al. (1986) Nature 324:73.
  7. Kestler, D.P. et al. (1995) Blood 86:4559.
  8. Kestler, D.P. et al. (1999) Am. J. Hematol. 61:169.
  9. Bihl, M.P. et al. (2002) Am. J. Respir. Cell Mol. Biol. 27:48.
  10. Alberti, L. et al. (2005) Cancer Res. 65:2.
  11. Murakami, M. et al. (1993) Science 260:1808.
  12. Muller-Newen, G. (2003) Sci. STKE 2003:PE40.
  13. Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125.
  14. Cerutti, A. et al. (1998) J. Immunol. 160:2145.

Long Name

Interleukin 6

Alternate Names

BSF-2, BSF2, IFNB2, IL6, MGI-2A

Entrez Gene IDs

3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)

Gene Symbol

IL6

UniProt

P05231.1

Additional IL-6 Products

Product Documents for Recombinant Human IL-6 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 206-IL (with carrier)
Download SDS 206-IL/CF (carrier free)

Product Specific Notices for Recombinant Human IL-6 Protein

For research use only

Citations for Recombinant Human IL-6 Protein

Customer Reviews for Recombinant Human IL-6 Protein (26)

4.7 out of 5
26 Customer Ratings
5 Stars
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4 Stars
23%
3 Stars
4%
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1 Stars
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Showing  1 - 5 of 26 reviews Showing All
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  • Recombinant Human IL-6 Protein
    Name: Lana Jagannathan
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 08/26/2024
    We use this cytokine as part of a dendritic cell maturation cocktail.
    Recombinant Human IL-6 Protein 206-IL
  • Recombinant Human IL-6 Protein
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 11/15/2023
    Recombinant Human IL-6 Protein 206-IL
  • Recombinant Human IL-6 Protein
    Name: Anonymous
    Application: Cell migration/motility
    Verified Customer | Posted 07/25/2023
    Recombinant Human IL-6 Protein 206-IL
  • Recombinant Human IL-6 Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 01/17/2022
    Recombinant Human IL-6 Protein 206-IL
  • Recombinant Human IL-6 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 08/07/2021
  • Recombinant Human IL-6 Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 05/24/2021
  • Recombinant Human IL-6 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 03/06/2021
  • Recombinant Human IL-6 Protein
    Name: Anonymous
    Application: Assay validation
    Verified Customer | Posted 02/09/2021
  • Recombinant Human IL-6 Protein
    Name: tehyeon kim
    Application: Cell Proliferation
    Verified Customer | Posted 01/17/2020
  • Recombinant Human IL-6 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 01/10/2020
  • Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 02/25/2019
  • Recombinant Human IL-6 Protein
    Name: Zoia Levashova
    Application: MSD assay
    Verified Customer | Posted 11/26/2018
    The recombinant human IL-6 protein was used as the calibration reagent in MSD assay with biotinylated MAB206 (capture) and Sulfo-Tag labeled AF-206-NA (detection) antibodies.
    Recombinant Human IL-6 Protein 206-IL
  • Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 10/08/2018
  • Recombinant Human IL-6 Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 05/11/2018
  • Recombinant Human IL-6 Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 03/02/2018
    Recombinant Human IL-6 Protein 206-IL
  • Recombinant Human IL-6 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 02/11/2018
  • Recombinant Human IL-6 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 06/28/2017
  • Name: Boubacar Barry
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 06/01/2017
  • Name: Anonymous
    Application: Cell migration/motility
    Verified Customer | Posted 05/23/2017
  • Recombinant Human IL-6 Protein
    Name: Michelle Chen
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 04/26/2017
  • Recombinant Human IL-6 Protein
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 12/15/2016
    Elevated levels of the proinflammatory cytokine IL-6 are associated with poor survival outcomes in many cancers. Antibodies targeting IL-6 and its receptor have been developed for chronic inflammatory disease, but they have not yet been shown to clearly benefit cancer patients, possibly due to antibody potency or the settings in which they have been tested. In this study, we describe the development of a novel high-affinity anti-IL-6 antibody, MEDI5117, which features an extended half-life and potent inhibitory effects on IL-6 biologic activity. MEDI5117 inhibited IL-6-mediated activation of STAT3, suppressing the growth of several tumor types driven by IL-6 autocrine signaling. In the same models, MEDI5117 displayed superior preclinical activity relative to a previously developed anti-IL-6 antibody. Consistent with roles for IL-6 in promoting tumor angiogenesis, we found that MEDI5117 inhibited the growth of endothelial cells, which can produce IL-6 and support tumorigenesis. Notably, in tumor xenograft assays.
  • Recombinant Human IL-6 Protein
    Name: Veronica Fregoso
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 08/01/2016
    Primary embryonic rat cortical cultures stimulated with IL-6 (20, 30, 40 ng/ml) for 30 minutes (PBS+BSA:negative stimulation control; Okadaic Acid, OA: positive phosphorylation control). Western blot for changes in Akt phosphorylation. *Note membranes for western were cut between 70kd and 50kd*
    Recombinant Human IL-6 Protein 206-IL
  • Recombinant Human IL-6 Protein
    Name: Kyle Blackshere
    Application: In vivo study
    Verified Customer | Posted 07/29/2016
  • Recombinant Human IL-6 Protein
    Name: deborah webb
    Application: Immunoassay Standard
    Verified Customer | Posted 05/16/2016
  • Recombinant Human IL-6 Protein
    Name: SOjan Abraham
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 01/21/2016
  • Recombinant Human IL-6 Protein
    Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 01/19/2016

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FAQs for Recombinant Human IL-6 Protein

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  • Q: Can Recombinant Human IL-6 protein (Catalog # 206-IL) be used on mouse cells?

    A: We evaluate the bioactivity of Recombinant Human IL-6 Protein (Catalog # 206-IL) in a cell proliferation assay using mouse plasmacytoma cells during QC testing. Catalog # 206-IL exhibits an ED50 in the range of 0.2-0.8 ng/mL in this assay using mouse cells. We have evaluated the human and mouse IL-6 proteins in the same assay and found the Recombinant Mouse IL-6 Protein (Catalog # 406-ML) was more active with mouse cells. This information can be found in the activity section on the respective product datasheets. 

  • Q: What are the differences between Recombinant Human IL-6 Protein (Catalog # 206-IL) and Recombinant Human IL-6 (HEK293-expressed) Protein (Catalog # 7270-IL)?

    A: Recombinant Human IL-6 Protein (Catalog # 206-IL) is raised in E. coli. The Recombinant Human IL-6 (HEK293-expressed) Protein (Catalog # 7270-IL) is raised in HEK293. Because #7270-IL is raised in mammalian cells, it is expected to have post translational modifications that are absent in the E. coli-derived version (206-IL). The HEK293-derived #7270-IL exhibits greater activity than the E. coli-derived #206-IL in the same activity assay.

  • Q: Can Recombinant Human IL-6 protein (Catalog # 206-IL) be used on mouse cells?

    A: We evaluate the bioactivity of Recombinant Human IL-6 Protein (Catalog # 206-IL) in a cell proliferation assay using mouse plasmacytoma cells during QC testing. Catalog # 206-IL exhibits an ED50 in the range of 0.2-0.8 ng/mL in this assay using mouse cells. We have evaluated the human and mouse IL-6 proteins in the same assay and found the Recombinant Mouse IL-6 Protein (Catalog # 406-ML) was more active with mouse cells. This information can be found in the activity section on the respective product datasheets. 

  • Q: What are the differences between Recombinant Human IL-6 Protein (Catalog # 206-IL) and Recombinant Human IL-6 (HEK293-expressed) Protein (Catalog # 7270-IL)?

    A: Recombinant Human IL-6 Protein (Catalog # 206-IL) is raised in E. coli. The Recombinant Human IL-6 (HEK293-expressed) Protein (Catalog # 7270-IL) is raised in HEK293. Because #7270-IL is raised in mammalian cells, it is expected to have post translational modifications that are absent in the E. coli-derived version (206-IL). The HEK293-derived #7270-IL exhibits greater activity than the E. coli-derived #206-IL in the same activity assay.

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