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Human Integrin  alpha M/CD11b Alexa Fluor® 594-conjugated Antibody

    
Datasheet / CoA / SDS
Detection of Integrin  alpha M/CD11b in Human PBMCs by Flow Cytometry.

Flow Cytometry

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  • Species Reactivity
    Human
  • Specificity
    Detects human Integrin alpha M/CD11b in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 238446
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Integrin alpha M/CD11b
    Phe17-Asn1105
    Accession # NP_001139280
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 594
Product Datasheets
Product Datasheet

COA

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SDS
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Flow Cytometry
Flow Cytometry CD11b Antibody (238446) [Alexa Fluor® 594]
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Detection of Integrin  alpha M/CD11b in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human Integrin  alpha M/CD11b Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # FAB16991T) or (B) Mouse IgG2B Alexa Fluor 594 Isotype Control (Catalog # IC0041T). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD11b/Integrin alpha M
The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha and a beta subunit. The Integrin alpha M subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin alpha M/ beta 2, also known as MAC-1 and Complement Receptor type 3 (CR3). Integrin alpha M/ beta 2 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alpha M/ beta 2 can bind several ligands (including ICAM-1, Fibrinogen and the C3 Complement Fragment C3bi) to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles.
  • Entrez Gene IDs:
    3684 (Human); 16409 (Mouse)
  • Alternate Names:
    antigen CD11b (p170); CD11 antigen-like family member B; CD11b antigen; CD11b; CD11Bintegrin, alpha M (complement component receptor 3, alpha; also known as CD11b(p170), macrophage antigen alpha polypeptide); Cell surface glycoprotein MAC-1 subunit alpha; CR-3 alpha chain; CR3AMGC117044; Integrin alpha M; integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; MAC-1; MAC1A; macrophage antigen alpha polypeptide; MO1A; neutrophil adherence receptor alpha-M subunit; Neutrophil adherence receptor; SLEB6
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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Alexa Fluor® 594-conjugated Isotype Control

 Ctrl IC0041T
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

 Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

 Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

 Flow FC002 1
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