Human Integrin alpha M/CD11b Alexa Fluor® 594-conjugated Antibody

FAB16991T Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB16991T-100
FAB16991T-025

Bulk Orders

Human Integrin alpha M/CD11b Alexa Fluor® 594-conjugated Antibody in Data

1 Image

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Integrin alpha M/CD11b Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Integrin alpha M/CD11b in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 238446

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Integrin alpha M/CD11b
Phe17-Asn1105
Accession # NP_001139280

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

5 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry

View Larger

Detection of Integrin alpha M/CD11b in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human Integrin aM/CD11b Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # FAB16991T) or (B) Mouse IgG_2BAlexa Fluor 594 Isotype Control (Catalog # IC0041T). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD11b/Integrin alpha M

The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha and a beta subunit. The Integrin alpha M subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin alpha M/ beta 2, also known as MAC-1 and Complement Receptor type 3 (CR3). Integrin alpha M/ beta 2 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alpha M/ beta 2 can bind several ligands (including ICAM-1, Fibrinogen and the C3 Complement Fragment C3bi) to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles.

Entrez Gene IDs

3684 (Human); 16409 (Mouse)

Alternate Names

antigen CD11b (p170); CD11 antigen-like family member B; CD11b antigen; CD11b; Cell surface glycoprotein MAC-1 subunit alpha; Complement component 3 receptor 3 subunit; CR-3 alpha chain; CR3A; CR3AMGC117044; Integrin alpha M; integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; MAC-1; MAC1A; macrophage antigen alpha polypeptide; MO1A; neutrophil adherence receptor alpha-M subunit; Neutrophil adherence receptor; SLEB6